冯伟红;李春;吉丽娜;傅欣桐;朱晶晶;陈两绵;杨立新;荣立新;张永欣;王智民;
FENG Wei-hong;LI Chun;JI Li-na;FU Xin-tong;ZHU Jing-jing;CHEN Liang-mian;YANG Li-xin;RONG Li-xin;ZHANG Yong-xin;WANG Zhi-min;Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences;National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines;Tianjin Institute for Drug Control;Beijing Institute For Drug Control;Guang' anmen Hospital,China Academy of Chinese Medical Sciences;

• 1:中国中医科学院中药研究所
• 2:中药质量控制技术国家工程实验室
• 3:天津市药品检验所
• 4:北京市药品检验所

摘要(Abstract)：

采用反相高效液相色谱法,Ultimate^C_(18)色谱柱,对红参中9种人参皂苷类成分进行了具有高分离度和高色谱峰纯度的同步色谱分离。采用"一测多评"中药质量评价模式,经一系列方法学考察,以人参皂苷Rb_1为内参物,建立了人参皂苷Rg_1,Re,Rf,Rb_1,Rc,Rb_2,Rb_3,Rd和20(S)-人参皂苷Rg3与人参皂苷Rb1间的相对校正因子与相对保留值,在红参对照图谱的辅助定位下,最终实现了仅采用人参皂苷Rb1一个中药对照品对红参中上述9种人参皂苷类成分的同步质量控制。
Using reversed-phase high performance liquid chromatography,nine ginsenosides were simultaneously separated on an Ultimate C_(18) column with high-resolution and high purity of each chromatographic peak. Adopting the QAMS quality evaluation model for traditional Chinese medicines,ginsenoside Rb_1 was used as the internal reference substance,and the relative correction factors(RCFs) and the relative retention values(RTRs) of ginsenosides Rg_1,Re,Rf,Rb_1,Rc,Rb_2,Rb_3,Rd and 20( S)-ginsenoside Rg_3 to ginsenoside Rb_1 were calculated individually. Through a series of methodology evaluations,and positioned by the red ginseng reference chromatograph and RTVs,nine ginsenosides in red ginseng were simultaneously assayed only by quantitative determined ginsenoside Rb_1.

关键词(KeyWords)： 红参;一测多评;高分离度;高色谱峰纯度;对照图谱;9种;人参皂苷
red ginseng;QAMS(qualitative assay of multi-components by a single reference substance);high-resolution;high purity;reference chromatogram;ginsenosides

Abstract:

Keywords:

基金项目(Foundation): 国家药品标准提高暨2015版药典科研任务;; 中药质量标准研究和信息化体系建设平台项目(2012ZX09304005);; 国家“重大新药创制”科技重大专项(2014ZX09304307001-002-007)

作者(Authors): 冯伟红;李春;吉丽娜;傅欣桐;朱晶晶;陈两绵;杨立新;荣立新;张永欣;王智民;
FENG Wei-hong;LI Chun;JI Li-na;FU Xin-tong;ZHU Jing-jing;CHEN Liang-mian;YANG Li-xin;RONG Li-xin;ZHANG Yong-xin;WANG Zhi-min;Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences;National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines;Tianjin Institute for Drug Control;Beijing Institute For Drug Control;Guang' anmen Hospital,China Academy of Chinese Medical Sciences;

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