中国中药杂志

2003, (01)

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高效液相色谱法测定不同产地葛根中葛根素、大豆苷及大豆苷元的含量
Determination of Puerarin, Daidzin and Daidzein in Root of Pueraria lobata of Different Origin by HPLC

金文姗,谈钰元,陈有根,王妍
JIN Wen-shan, TAN Yu-yuan, CHEN You-gen, WANG Yan (Beijing Yuke Botanical Development CO., LTD., Beijing 100062, China)

摘要(Abstract):

目的 :建立同时测定葛根中葛根素、大豆苷及大豆苷元的含量测定方法。方法 :选用SYMMETRY C18色谱柱 (3.9mm× 15 0mm ,5 μm) ,甲醇 1%醋酸水溶液为流动相梯度洗脱 ,紫外检测波长为 2 5 0nm ,HPLC法。结果 :平均回收率分别为 10 1.7% ,10 0 .7% ,10 1.7% (n =3) ,RSD分别为 0 .43 % ,0 .82 % ,1.5 0 % (n =3)。结论 :本法简便 ,准确 ,快速 ;适用于含葛根素、大豆苷及大豆苷元的葛根药材及制剂的质量控制。
Objective: To develop an HPLC method to determine Puerarin, Daidzin and Daidzein in Pueraria lobata . Method: The separation was performed in a SYMMETRY C 18 column with a mobile phase of methanol-1%HAC solution (gradient elution), the The detection wavelength being 250 nm. Result: The average recovery was respectively 101.7%,100.7% and 101.7%( n =3),RSD 0.43%, 0.82% and 1.50%( n =3) for Puerarin,Daidzin and Daidzein. Conclusion: This method is suitable for the determination of Puerarin,Daidzin and Daidzein in Pueraria lobata and its preparation.

关键词(KeyWords): 二极管阵列高效液相色谱法;梯度洗脱;葛根素;大豆苷;大豆苷元;野葛
HPLC with DAD detection; gradient elution; Puerarin; Daidzin; Daidzein; Pueraria lobata

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作者(Author): 金文姗,谈钰元,陈有根,王妍
JIN Wen-shan, TAN Yu-yuan, CHEN You-gen, WANG Yan (Beijing Yuke Botanical Development CO., LTD., Beijing 100062, China)

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