中国中药杂志

2019, v.44(12) 2480-2485

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丹参酮生物合成途径CYP450基因相关长链非编码RNA的筛选
Screening of long non-coding RNA related to CYP450s involved in biosynthesis of tanshinones

蔡媛;马莹;郭娟;王勇庆;杜青;陈同;张水寒;黄璐琦;
CAI Yuan;MA Ying;GUO Juan;WANG Yong-qing;DU Qing;CHEN Tong;ZHANG Shui-han;HUANG Lu-qi;Institute of Traditional Chinese Medicine,Hunan Academy of Traditional Chinese Medicine;State Key Laboratory Breeding Base of Dao-di Herbs,National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences;

摘要(Abstract):

丹参酮是中药丹参的主要有效成分,其生物合成途径表明细胞色素CYP450酶在丹参酮生物合成途径结构后修饰过程中发挥着重要作用。长链非编码RNA(lncRNA)是一类长度大于200 nt的核苷酸,对药用植物的生长发育、次生代谢等具有重要调控作用。该研究通过对丹参毛状根进行诱导,利用高通量测序构建其长链非编码RNA文库,共获得8 942个差异lncRNA,其中6 755个基因间lncRNA,并鉴定出1 115 814个lncRNA-靶基因对,包括122个lncRNA-靶基因顺式对。对差异lncRNA与CYP450进行相关性分析,共鉴定出16 249个lncRNA-CYP450靶基因对。将其与丹参酮生物合成途径CYP76AH1,CYP76AH3,CYP76AK1基因进行靶向相关性分析,得到216个靶基因,这些候选基因为丹参酮生物合成途径下游调控机制研究奠定基础。
Tanshinones are abietane-type norditerpenoid quinones that make up the main bioactive ingredients of traditional Chinese medicine Salvia miltiorrhiza. Cytochrome CYP450 plays an important role in the post-structural modification of tanshinone biosynthesis pathway. Long non-coding RNA( lncRNA) have been defined as transcripts longer than 200 nucleotides,which have been functionally characterized in regulating the growth and development,secondary metabolism and stress of medicinal plants. In this study,we perform a comprehensive identification of lncRNAs in response to tanshinone metabolism induced by yeast extract( YE) and Ag~+ S. miltiorrhiza hairy roots. Deep RNA sequencing was used to identify a set of different 8 942 lncRNAs,of which 6 755 were intergenic lncRNAs. We predicted a total of 1 115 814 lncRNA-coding gene pairs,including 122 lncRNA-coding gene as cis pairs. The correlation analysis between lncRNA and CYP450 related to tanshinone biosynthesis was carried out and a total of 16 249 lncRNA-CYP450 target gene pairs were identified. Further analysis with functional known CYP76 AH1,CYP76 AH3 and CYP76 AK1 involved in tanshinone biosynthesis,we also identified a set of 216 target genes. These candidate genes will be the important target in the downstream regulation mechanism analysis of the tanshinone biosynthesis pathway.

关键词(KeyWords): 丹参;丹参酮;长链非编码RNA;CYP450
Salvia miltiorrhiza;tanshinone;long non-coding RNA;cytochrome P450

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金青年基金项目(81503197,81573532.81822046);; 中央本级重大增减支项目(2060302);; 湖南省自然科学基金青年基金项目(2018JJ3310);; 湖南省中医药管理局项目(201810);; 湖湘中药资源保护与利用2011协同创新中心项目

作者(Author): 蔡媛;马莹;郭娟;王勇庆;杜青;陈同;张水寒;黄璐琦;
CAI Yuan;MA Ying;GUO Juan;WANG Yong-qing;DU Qing;CHEN Tong;ZHANG Shui-han;HUANG Lu-qi;Institute of Traditional Chinese Medicine,Hunan Academy of Traditional Chinese Medicine;State Key Laboratory Breeding Base of Dao-di Herbs,National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences;

Email:

DOI: 10.19540/j.cnki.cjcmm.20190324.101

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