中国中药杂志

2006, (09) 766-769

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复方丹参片对冠心病患者血浆蛋白影响的蛋白质组学研究
Effects of fufangdenshen tablets on human atherosclerotic plasma protein using proteomic technique

马增春;高月;谭洪玲;肖成荣;吴红金;王升启;
MA Zeng-chun~(1),GAO Yue~(1),TAN Hong-ling~(1),XIAO Cheng-rong~(1),WU Hong-jin~(2),WANG Sheng-qi~(1)(1.Institute of Radiation Medicine,Beijing 100850,China;2.Beijing Hospital of United Chiniese-Western Medicine,Beijing 100039,China)

摘要(Abstract):

目的:用蛋白质组学技术考察复方丹参片对冠心病患者血浆蛋白的影响,在分子水平上探讨复方丹参片活血化瘀的作用机制。方法:收集正常、冠心病患者和复方丹参片用药病人组的血浆,用二向电泳、图像分析、质谱鉴定等蛋白质组学技术测定复方丹参片对冠心病患者血浆中有影响的蛋白质。结果:蛋白质组学研究发现,冠心病病人血浆与正常相比有3个蛋白质下调和6个蛋白质上调,复方丹参片可使这些改变的蛋白质水平有所恢复。胶内酶切提取蛋白进行质谱鉴定,其中冠心病病人血浆与正常相比升高的蛋白质有免疫球蛋白、纤维蛋白原、粒酶,冠心病病人血浆与正常相比降低的蛋白质有细胞表面糖蛋白(CD44SP)。冠心病病人服用复方丹参片后可降低免疫球蛋白、纤维蛋白原、粒酶。结论:复方丹参片可以降低冠心病患者血浆中的纤维蛋白原和粒酶,其可能通过抑制单核细胞黏附、平滑肌细胞增殖和迁移、减轻炎症而治疗冠状动脉粥样硬化。
Objective: To research the effects of fufangdenshen tablets on atherosclerotic plasma protein using proteomic technique and further explore its potential molecular mechanism to cure atherosclerosis.Method: The plasma of normal,atherosclerosis and cured group were collected,and the albumin in plasma was removed.Proteomic protocol involved of 2DE,image analysis and spectrometry detection was used to detect regulated plasma protein by fufangdenshen tablets.Result: 3 decreased expressed and 6 higher protein in atherosclerotic plasma could be recovered by fufangdenshen tablets.The levels of fibrinogen,Granzyme C and immunoglobulin were decreased by fufangdenshen tablets. Conclusion: The levels of fibrinogen,Granzyme C in atherosclerotic plasma could be decreased by fufangdenshen tablets.The effects of fufangdenshen tablets on atherosclerosis included that: inhibition of adhesion of monocyte,inhibition of proliferation and migration of VSMC,and weakening of inflammation.

关键词(KeyWords): 复方丹参片;蛋白质组学;动脉粥样硬化;冠心病
fufangdenshen tablets;proteomics;atherosclerosis;coronary heart disease

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基金项目(Foundation): 国家863重大专项(2003AA2Z2042);; 国家自然科学基金资助项目(30271617)

作者(Author): 马增春;高月;谭洪玲;肖成荣;吴红金;王升启;
MA Zeng-chun~(1),GAO Yue~(1),TAN Hong-ling~(1),XIAO Cheng-rong~(1),WU Hong-jin~(2),WANG Sheng-qi~(1)(1.Institute of Radiation Medicine,Beijing 100850,China;2.Beijing Hospital of United Chiniese-Western Medicine,Beijing 100039,China)

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