张昭;夏天睿;陶月红;戴灵超;刘琰璐;张本刚;
ZHANG Zhao,XIA Tianrui,TAO Yuehong,DAI Lingchao,LIU Yanlu,ZHANG Bengang(Institute of Medicinal Plant Development,Peking Union Medical College and Chinese Academyof Medical Sciences,Beijing 100193,China)

• 1:中国医学科学院北京协和医学院药用植物研究所

摘要(Abstract)：

目的:通过对黄檗果皮化感作用的研究,探讨其在黄檗野生种群自我更新障碍中的作用,为黄檗野生资源的保护寻求方法和途径。方法:用4℃蒸馏水浸泡(水浸液)及蒸馏水回流提取(水提液)的黄檗果皮提取液培养白菜、小麦种子,研究其对受体种子萌发及幼苗生长的作用。结果:20 g.L-1水浸液及水提液对白菜、小麦种子的萌发有显著的抑制作用,100g.L-1时完全抑制小麦的萌发;20 g.L-1水浸液显著抑制白菜、小麦的生长,完全抑制白菜根的生长,100 g.L-1水浸液完全抑制白菜、小麦的生长;相比于水浸液,水提液对种子萌发及幼苗生长的作用强度都有所减弱。结论:黄檗果皮的化感作用是多物质作用的结果,部分化感物质对热敏感;黄檗果皮的化感作用总体上表现为对种子萌发及幼苗生长的抑制,推测黄檗果皮的物理及化学特性是致使其种群自我更新困难的因素之一。
Through the study of allelopathy of the pericarp of Phellodendron amurense,the role of self-regeneration barriers was investigated in order to find ways and means for the protection of wild populations of P.amurense.Solution preparation: soaked pericarp of P.amurense in distilled water at 4 ℃ to get solution A,and reflux extraction of pericarp with distilled water at 100 ℃ to get solution B.Both of the solution A and solution B were used in the experiment of seed germination and seedling growth with the seeds of cabbage and wheat.The results showed that 20 g·L-1 concentration of solution A and solution B inhibited significantly seed germination of cabbage and wheat,while 100 g·L-1 concentration of solution A even completely inhibited the seed germination of wheat.20 g·L-1 concentration of solution A significantly inhibited the cabbage and wheat seedling growth,completely inhibited the root growth of cabbage,while 100 g·L-1 concentrations of solution A completely inhibited seedling growth of cabbage and wheat.Comparing to solution A,the intensity of solution B are diminished on seed germination and seedling growth.It is concluded that the allelopathy of pericarp of P.amurense is multi-material role in the results,some of allelochemicals are easily degradable when exposed to heat.Overall,the allelopathy of pericarp of P.amurense can affect the seed germination and seedling growth.It is supposed that allelochemicals existed in the pericarp of P.amurense is one of the reason leading to difficulties in self-regeneration of its population.

关键词(KeyWords)： 黄檗;关黄柏;致危因素;化感作用;活性检测
Phellodendron amurense;Cortex Phellodendri Amurene;endangered factors;allelopathy;bioassay

Abstract:

Keywords:

基金项目(Foundation): 国家“重大新药创制”科技重大专项(2009ZX09308-002)

作者(Authors): 张昭;夏天睿;陶月红;戴灵超;刘琰璐;张本刚;
ZHANG Zhao,XIA Tianrui,TAO Yuehong,DAI Lingchao,LIU Yanlu,ZHANG Bengang(Institute of Medicinal Plant Development,Peking Union Medical College and Chinese Academyof Medical Sciences,Beijing 100193,China)

参考文献(References)：

• [1]Min An,D LLiu,I R Johnson,et al.Mathematical modelling ofallelopathy:II.The dynamics of allelochemicals from livingplants in the environment[J].Ecol Model,2003,161:53.
• [2]Molisch H.Der Einfluss einer pflanze auf die andere-allelopathie[M].Jena:Gustav Fischer Verlag,1937:13.
• [3]Harborne J B.Chemical signals in the ecosystem[J].Ann Bot,1987,60:39.
• [4]Lovett J V,Ryuntyu M Y,Garlick P R.Allelopathic effects ofthornapple,Datura stramoniumL.[C].Sydney:Proceedings ofthe Eighth Australian Weeds Conference,1987:179.
• [5]A L Viles,R N Reese.Allelopathic potential ofEchinacea an-gustifoliaD.C.[J].Environ Exp Bot,1996,36(1):39.
• [6]傅立国.中国植物红皮书——稀有濒危植物.第1册[M].北京:科学出版社,1991:572.
• [7]夏天睿,张昭,张本刚,等.稀有濒危常用中药材保护现状评述[J].世界科学技术——中医药现代化,2005,8(6):115.
• [8]常家传.自然生态系统中的斑鸫与黄檗[J].生态学杂志,2000,19(1):70.
• [9]鲁长虎,常家传,许青,等.黄檗的更新特点及食果实鸟类对其种子的传播[J].生态学杂志,2004,23(1):24.
• [10]Shinsuke Fujihara,Tokurou Shimizu.Growth inhibitory effect ofpeel extract fromCitrus junos[J].Plant Growth Regul,2003,39:22.
• [11]Willamson G B.Biossays for allelopathy:measuring treatment re-sponces with independent on controlls[J].J Chem Ecol,1988,14(1):181.
• [12]夏天睿,张昭,张本刚,等.中国植物园.第11期[M].北京:中国林业出版社,2006:95.