陈佳敏;汪涛;郭巧生;李浩文;左琳;邹庆军;杨锋;
CHEN Jia-min;WANG Tao;GUO Qiao-sheng;LI Hao-wen;ZUO Lin;ZOU Qing-jun;YANG Feng;Institute of Chinese Medicinal Materials,Nanjing Agricultural University;

• 1:南京农业大学中药材研究所

摘要(Abstract)：

为探讨熵权TOPSIS法在野菊花提取液对DPPH,ABTS,羟基自由基的清除作用以及对黄嘌呤氧化酶(XOD)和脂肪氧化酶(LOX)的抑制作用比较的应用。以31个不同产地的野菊花为研究对象,采用DPPH法、ABTS法、水杨酸法和分光光度法测定不同产地野菊花提取液的对DPPH,ABTS,羟基自由基的清除作用以及对XOD和LOX的抑制作用。以清除DPPH,ABTS,羟基自由基以及对XOD和LOX的抑制作用的半数抑制率为评价指标,主成分分析法提取了2个主成分,累计贡献率达到了92.4%,同时将31个不同产地野菊花分为大别山区和秦岭-太行山2类。采用熵权TOPSIS法对各项指标客观赋权,以相对接近度(C_i)为测度综合排序,构建野菊花多指标综合评价模型。结果表明,31个不同产地的野菊花相似接近度的大小在0.098～0.983,不同产地的野菊花提取液在清除DPPH,ABTS和羟基自由基以及对XOD,LOX的抑制作用上具有显著差异。其中大别山地区野菊花测得相似接近度大,质量排序靠前。指标权重系数以及产区分类结果与主成分分析结果基本一致。基于熵权赋权法的TOPSIS法可用于野菊花的综合质量评价。
The study is aiming at investigating the application of entropy weight TOPSIS method in the comparison of the scavenging effect of DPPH, ABTS and hydroxyl radical and the inhibition effect of xanthine oxidase(XOD) and lipoxygenase(LOX) of Chrysanthemum indicum. The DPPH, ABTS, salicylic acid and spectrophotometry were used to determine the scavenging effect of DPPH, ABTS and hydroxyl radical and the inhibition effect of xanthine oxidase(XOD) and lipoxygenase(LOX) of Ch. indicum from 31 different areas in vitro. Take the half inhibition rate of as the evaluation index, two principal components were extracted by the principal component analysis, and their cumulative contribution rate reached at 92.4%. The different areas of Ch. indicum could be divided into Dabei Mountain and Qinling-Taihang Mountain by use principal component to analysis. The entropy weight TOPSIS method was used to objectively assign weights to five indexes, calculate the weight of each index and set up the best and worst scheme of the evaluation object, and the relative proximity(C_i) was used as the measure to construct the multi-index comprehensive evaluation model of Ch. indicum. And then sort with the relative proximity value. The results showed that the relative proximity was between 0.098 and 0.983 which represents there were significant differences in the scavenging effect of DPPH, ABTS and hydroxyl radical and the inhibition effect of xanthine oxidase(XOD) and lipoxygenase(LOX) between extracts of Ch. indicum from different areas. The Ch. indicum from Dabie Mountain area have a relatively high relative degree of measurement and high-quality ranking. Taken together, the quality of Ch. indicum.from the Dabie Mountain area is better. The index weight coefficient and the classification result of producing area are basically consistent with the result of principal component analysis. The results show that the TOPSIS method based on entropy weight method can be used to evaluate the comprehensive quality of Ch. indicum.

关键词(KeyWords)： 野菊花;抗氧化活性;抗炎活性;熵权TOPSIS法;主成分分析
Chrysanthemum indicum;antioxidant activity;anti-inflammatory activity;entropy weight and TOPSIS methodology;principal component analysis

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金青年基金项目(81503180);; 国家药品标准提高项目(2017-556);; 江苏现代农业产业技术体系建设专项(JATS[2019]426)

作者(Authors): 陈佳敏;汪涛;郭巧生;李浩文;左琳;邹庆军;杨锋;
CHEN Jia-min;WANG Tao;GUO Qiao-sheng;LI Hao-wen;ZUO Lin;ZOU Qing-jun;YANG Feng;Institute of Chinese Medicinal Materials,Nanjing Agricultural University;

DOI: 10.19540/j.cnki.cjcmm.20201122.102

参考文献(References)：

• [1] 国家药典委员会.中华人民共和国药典.一部[S].北京:中国医药科技出版社,2015:314.
• [2] 陈丹丹,邹庆军,郭巧生,等.短日照处理对野菊CO基因表达量的影响[J].中国中药杂志,2019,44(4):648.
• [3] 中国科学院中国植物志编辑委员会.中国植物志[M].北京:科学出版社,1983.
• [4] 郭巧生,房海灵,申海进.不同产地野菊花中绿原酸、咖啡酸和蒙花苷含量[J].中国中药杂志,2010,35(9):1160.
• [5] 周虹云,吴长顺,程存归.野菊花化学成分研究[J].中国现代应用药学,2013,30(1):31.
• [6] 张瑶,高慧,王新杰,等.熵权赋权法的灰色系统理论在当归饮片质量评价中的应用研究[J].中国中药杂志,2020,45(21):5200.
• [7] 冯伟红,李春,信伟梅,等.生物测定法用于中药质量评价的探索研究——以夏枯草抗氧化活性与总酚酸含量相关性的研究为例[J].中国中药杂志,2016,41(14):2660.
• [8] 李鸣真,叶望云,陆付耳.中医“清热解毒法”实质的研究[J].浙江中西医结合杂志,2000,10(8):45.
• [9] 姚入宇,陈兴福,艾莉,等.TOPSIS法综合比较不同采收期青川产北柴胡种子产量质量[J].中国中药杂志,2013,38(24):4271.
• [10] 顾志荣,王亚丽,孙宇靖,等.熵权TOPSIS模型在甘肃产当归药材综合质量评价中的应用[J].中药材,2014,37(9):1549.
• [11] 马天翔,顾志荣,许爱霞,等.基于OPLS结合熵权TOPSIS法对不同产地锁阳的鉴别与综合质量评价[J].中草药,2020,51(12):3284.
• [12] MISHRA K,OJHA H,CHAUDHURY N K.Estimation of antiradical properties of antioxidants using DPPH assay:a critical review and results[J].Food Chem,2012,130(4):1036.
• [13] 王罗玲,沈雪林,李亚峰,等.香青菜不同溶剂提取物抗氧化活性研究[J].食品研究与开发,2017,38(9):28.
• [14] SMIRNOFF N,CUMBES Q J.Hydroxyl radical scavenging activity of compatible solutes[J].Phytochemistry,1989,28(4):1057.
• [15] 李英,陈君,李萍.金银花中酚酸类和黄酮类成分的黄嘌呤氧化酶抑制活性[J].中国药科大学学报,2011,42(5):407.
• [16] 吴宛澐,陶新,徐子伟.桂枝等18种中药材水提液对5-脂肪氧化酶(5-LOX)活性的抑制作用[J].浙江农业学报,2017,29(1):31
• [17] 石岩,魏锋,马双成.关于主成分分析在中药和天然药物分析研究中应用的探讨[J].中国中药杂志,2018,43(14):3031.
• [18] 褚福红,于新.野菊花提取物清除自由基作用的研究[J].食品工业,2010,31(5):1.
• [19] DEBNATH T,JIN H L,HASNAT M A,et al.Antioxidant potential and oxidative dna damage preventive activity of Chrysanthemum indicum extracts [J].Food Biochem,2013,37(4):440.
• [20] 郑继标,吴晓丽,梁丽梅,等.野菊花超临界二氧化碳萃取物的镇痛抗炎作用研究[J].今日药学,2016,26(3):149.
• [21] 张金杰,吕文文,孙迎东,等.野菊花中黄酮类成分抗炎活性的虚拟筛选[J].药学研究,2018,37(10):565.
• [22] 张骏艳,张磊,李俊,等.野菊花总黄酮对佐剂性关节炎大鼠氧自由基代谢的影响[J].中国中药杂志,2010,35(3):344.
• [23] KONG L D,CAI Y,HUANG W W,et al.Inhibition of xanthine oxidase by some Chinese medicinal plants used to treat gout [J].J Ethnopharmacol,2000,73:199.