中国中药杂志

2021, v.46(04) 907-914

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基于熵权TOPSIS法对不同产地野菊花醇提物抗氧化性抗炎活性的比较
Comprehensive antioxidant and anti-inflammatory activity of alcohol extracts from Chrysanthemum indicum in different areas based on entropy weight and TOPSIS methodology

陈佳敏;汪涛;郭巧生;李浩文;左琳;邹庆军;杨锋;
CHEN Jia-min;WANG Tao;GUO Qiao-sheng;LI Hao-wen;ZUO Lin;ZOU Qing-jun;YANG Feng;Institute of Chinese Medicinal Materials,Nanjing Agricultural University;

摘要(Abstract):

为探讨熵权TOPSIS法在野菊花提取液对DPPH,ABTS,羟基自由基的清除作用以及对黄嘌呤氧化酶(XOD)和脂肪氧化酶(LOX)的抑制作用比较的应用。以31个不同产地的野菊花为研究对象,采用DPPH法、ABTS法、水杨酸法和分光光度法测定不同产地野菊花提取液的对DPPH,ABTS,羟基自由基的清除作用以及对XOD和LOX的抑制作用。以清除DPPH,ABTS,羟基自由基以及对XOD和LOX的抑制作用的半数抑制率为评价指标,主成分分析法提取了2个主成分,累计贡献率达到了92.4%,同时将31个不同产地野菊花分为大别山区和秦岭-太行山2类。采用熵权TOPSIS法对各项指标客观赋权,以相对接近度(C_i)为测度综合排序,构建野菊花多指标综合评价模型。结果表明,31个不同产地的野菊花相似接近度的大小在0.098~0.983,不同产地的野菊花提取液在清除DPPH,ABTS和羟基自由基以及对XOD,LOX的抑制作用上具有显著差异。其中大别山地区野菊花测得相似接近度大,质量排序靠前。指标权重系数以及产区分类结果与主成分分析结果基本一致。基于熵权赋权法的TOPSIS法可用于野菊花的综合质量评价。
The study is aiming at investigating the application of entropy weight TOPSIS method in the comparison of the scavenging effect of DPPH, ABTS and hydroxyl radical and the inhibition effect of xanthine oxidase(XOD) and lipoxygenase(LOX) of Chrysanthemum indicum. The DPPH, ABTS, salicylic acid and spectrophotometry were used to determine the scavenging effect of DPPH, ABTS and hydroxyl radical and the inhibition effect of xanthine oxidase(XOD) and lipoxygenase(LOX) of Ch. indicum from 31 different areas in vitro. Take the half inhibition rate of as the evaluation index, two principal components were extracted by the principal component analysis, and their cumulative contribution rate reached at 92.4%. The different areas of Ch. indicum could be divided into Dabei Mountain and Qinling-Taihang Mountain by use principal component to analysis. The entropy weight TOPSIS method was used to objectively assign weights to five indexes, calculate the weight of each index and set up the best and worst scheme of the evaluation object, and the relative proximity(C_i) was used as the measure to construct the multi-index comprehensive evaluation model of Ch. indicum. And then sort with the relative proximity value. The results showed that the relative proximity was between 0.098 and 0.983 which represents there were significant differences in the scavenging effect of DPPH, ABTS and hydroxyl radical and the inhibition effect of xanthine oxidase(XOD) and lipoxygenase(LOX) between extracts of Ch. indicum from different areas. The Ch. indicum from Dabie Mountain area have a relatively high relative degree of measurement and high-quality ranking. Taken together, the quality of Ch. indicum.from the Dabie Mountain area is better. The index weight coefficient and the classification result of producing area are basically consistent with the result of principal component analysis. The results show that the TOPSIS method based on entropy weight method can be used to evaluate the comprehensive quality of Ch. indicum.

关键词(KeyWords): 野菊花;抗氧化活性;抗炎活性;熵权TOPSIS法;主成分分析
Chrysanthemum indicum;antioxidant activity;anti-inflammatory activity;entropy weight and TOPSIS methodology;principal component analysis

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金青年基金项目(81503180);; 国家药品标准提高项目(2017-556);; 江苏现代农业产业技术体系建设专项(JATS[2019]426)

作者(Author): 陈佳敏;汪涛;郭巧生;李浩文;左琳;邹庆军;杨锋;
CHEN Jia-min;WANG Tao;GUO Qiao-sheng;LI Hao-wen;ZUO Lin;ZOU Qing-jun;YANG Feng;Institute of Chinese Medicinal Materials,Nanjing Agricultural University;

Email:

DOI: 10.19540/j.cnki.cjcmm.20201122.102

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