中国中药杂志

2014, v.39(04) 715-720

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中药秦艽和习用品中5种环烯醚萜类成分的HPLC含量测定
Simultaneous determination of five iridoids in Gentianae Macrophyllae Radix and their local variety by HPLC

吴靳荣;吴立宏;赵志礼;王峥涛;
WU Jin-rong;WU Li-hong;ZHAO Zhi-li;WANG Zheng-tao;Department of Pharmacognosy,Shanghai University of Traditional Chinese Medicine;The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key Laboratoryfor New Resources and Quality Evaluation of Chinese Medicines,Institute of Chinese Materia Medica,Shanghai University of Traditional Chinese Medicine;

摘要(Abstract):

环烯醚萜类成分是中药秦艽的主要活性成分,其中马钱苷酸、6'-O-β-D-葡萄糖基龙胆苦苷、獐牙菜苦苷、龙胆苦苷、獐牙菜苷在秦艽药材中的含量相对较高且具有良好的药理活性。实验中建立了中药秦艽中5种环烯醚萜的含量测定方法:Shiseido SPOLAR C_(18)色谱柱(4.6 mm×250 mm,5μm);流动相为0.04%甲酸水(A)-乙腈(B)梯度洗脱;体积流量0.8 mL·min~(-1);检测波长240 nm;柱温30℃;进样量5μL。该方法分离度良好,标准曲线在线性范围内相关性良好,加样回收率均值在97.35%~106.2%。对8种39份秦艽药材进行含量测定,结果显示5种环烯醚萜类成分在8种秦艽类药材中均有分布。种内含量差异较大表明含量除了与物种有关外,与种质资源、环境和生长年限都密切相关。小秦艽中各组分的含量明显低于其他3种正品秦艽。
This study aims to establish a new method for quality evaluation of Gentianae Macrophyllae Radix by simultaneous determination of five iridoids(loganic acid,6'-O-β-D-glucopyranosylgentiopicroside,swertiamarin.gentiopicroside,sweroside),and to detect five iridoids in the root of eight species(Gentiana macrophylla,G.straminea,G.crassicaulis,G.dahurica,G.robusta,G.waltonii,G.lhassica,and G.tibetica).The separation was carried out on a Shiseido SPOLAR C_(18)(4.6 mm ×250 mm,5 μm)column eluted with mobile phase of water containing 0.04%formic acid(A) and acetonitrile(B) in a gradient program.The flow rate was 0.8 mL·min~(-1).The detect wavelength was set at 240 nm.The column temperature was kept at 30 ℃.The volume of injection was 5 μL.The five iridoids were well separated with ideal linear correlations.The average recoveries were 97.35%-106.23%.All the five iridoids were detected in the root of eight species.The contents of same species changed in a somewhat wider range.The contents in root of G.dahurica were lower than that in other species.

关键词(KeyWords): 秦艽;HPLC;环烯醚萜
Gentianae Macrophyllae Radix;HPLC;iridoids

Abstract:

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基金项目(Foundation): 国家自然科学基金面上项目(81173654);; 上海市科委一般项目(10ZR1429800)

作者(Author): 吴靳荣;吴立宏;赵志礼;王峥涛;
WU Jin-rong;WU Li-hong;ZHAO Zhi-li;WANG Zheng-tao;Department of Pharmacognosy,Shanghai University of Traditional Chinese Medicine;The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key Laboratoryfor New Resources and Quality Evaluation of Chinese Medicines,Institute of Chinese Materia Medica,Shanghai University of Traditional Chinese Medicine;

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