中国中药杂志

2016, v.41(24) 4635-4641

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黄蜀葵花中黄酮类成分对前脂肪细胞增殖、分化及胰岛素抵抗的影响
Effect of flavonoids from Abelmoschus manihot on proliferation,differentiation of 3T3-L1 preadipocyte and insulin resistance

蔡红蝶;宿树兰;郭盛;朱悦;钱大玮;陶伟伟;段金廒;
CAI Hong-die;SU Shu-lan;GUO Sheng;ZHU Yue;QIAN Da-wei;TAO Wei-wei;DUAN Jin-ao;Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization,and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine,Nanjing University of Chinese Medicine;Center for Translational Systems Biology and Neuroscience,Laboratory of Integrative Biomedicine of Brain Diseases,School of Basic Biomedical Science,Nanjing

摘要(Abstract):

黄蜀葵花中富含黄酮类成分,具有保护心血管、改善肾功能等作用。该文采用3T3-L1前脂肪细胞诱导分化的成熟脂肪细胞,建立胰岛素抵抗模型,分为正常组,模型组,异槲皮苷(HY)、金丝桃苷(JY)、槲皮素(QT)、槲皮素-3'-O-葡萄糖苷(QG)、棉皮素-8-O-β-葡萄糖醛酸(GG)给药组,BCA法检测细胞培养液中葡萄糖的含量,荧光定量q PCR检测PPARγ,C/EBPα,SREBP-1,脂联素,内酯素,抵抗素基因mRNA的表达水平。结果显示,5种黄酮类化合物5μmol·L-1浓度时可促进3T3-L1前脂肪细胞的增殖,浓度为100μmol·L-1时可抑制前脂肪细胞的增殖;与正常组比较,模型组细胞对葡萄糖摄取量降低(P<0.01);与模型组相比,5种黄酮类化合物100μmol·L-1给药组细胞葡萄糖消耗量显著上升(P<0.01),PPARγ,C/EBPα,脂联素表达明显增加(P<0.01),SREBP-1,抵抗素,内酯素的表达明显降低(P<0.01),促使脂肪细胞分化。研究表明,HY,JY,QT,QG,GG能调控前脂肪细胞增殖,促进脂肪细胞分化及糖脂代谢相关因子PPARγ,C/EBPα,SREBP-1,脂联素,内酯素,抵抗素的表达,促进前脂肪细胞分化,增加葡萄糖利用,从而改善胰岛素抵抗。
Abelmoschus manihot was rich in flavonoids,which has been reported the activity on protecting angiocarpy and improving renal function. This study aimed to explore the action mechanism of five flavonoids from A. manihot on how to ameliorating insulin resistance through the regulation of the glucose and expression of PPARγ,C/EBPα,SREBP-1,resistin,visfatin,adiponectin in 3 T3-L1 adipocytes. After the 3 T3-L1 preadipocytes were differentiated into mature adipocytes,insulin resistance model was built. Insulin resistance adipocytes were treated with 5,100 μmol· L-1quercetin,isoquercitrin,hyperoside,quercitrin-3 '-O-glucoside,gossypetin-8-O-β-glucoside. The glucose was indirectly determined by BCA kit. The mRNA expression levels of PPARγ,C/EBPα,SREBP-1,resistin,visfatin,adiponectin were detected by real-time quantitative PCR. Results showed that five flavonoids at 5 μmol·L-1could accelerate preadipocytes proliferation and inhibit that at 100 μmol·L-1Compared with the normal group,glucose uptake reduced significantly in model group( P < 0. 01). With the treatment of five flavonoids at 100 μmol·L-1,glucose consumption increased significantly( P < 0. 01). The high expression of PPARγ,C/EBPα,adiponectin expression was significantly increased( P < 0. 01),and low expression of SREBP-1,resistin,visfatin after respective administration with five flavonoids at 100 μmol·L-1promoted adipocyte differentiation. This study showed that,HY,JY,QT,QG,GG can control preadipocytes proliferation,promote adipocyte differentiation and regulate the expression of relative factors with lipid metabolism,such as PPARγ,C/EBPα,SREBP-1,adiponectin,resistin,visfatin,increasing glucose utilization and improving insulin resistance in 3 T3-L1 adipocyte.

关键词(KeyWords): 锦葵科;黄属葵花;胰岛素抵抗;黄酮类成分;3T3-L1脂肪细胞
Malvaceae;Abelmoschus manihot;insulin resistance;flavonoids;3T3-L1 adipocyte

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基金项目(Foundation): 国家自然科学基金项目(81373889,81673533);; 江苏省中药学优势学科Ⅱ期建设项目(ysxk-2014);; 2013年国家教育部“新世纪优秀人才”计划项目(NCET-13-0873);; 江苏省“333高层次人才培养工程”项目(BRA2015391)

作者(Author): 蔡红蝶;宿树兰;郭盛;朱悦;钱大玮;陶伟伟;段金廒;
CAI Hong-die;SU Shu-lan;GUO Sheng;ZHU Yue;QIAN Da-wei;TAO Wei-wei;DUAN Jin-ao;Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization,and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine,Nanjing University of Chinese Medicine;Center for Translational Systems Biology and Neuroscience,Laboratory of Integrative Biomedicine of Brain Diseases,School of Basic Biomedical Science,Nanjing

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