中国中药杂志

2022, v.47(18) 5032-5039

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

蛹虫草抗非小细胞肺癌的血清代谢组学研究
Mechanism of Cordyceps militaris against non-small cell lung cancer: based on serum metabolomics

陆颖颖;黄枭;罗子宸;祁明媛;单进军;张雯;狄留庆;
LU Ying-ying;HUANG Xiao;LUO Zi-chen;QI Ming-yuan;SHAN Jin-jun;ZHANG Wen;DI Liu-qing;School of Pharmacy, Nanjing University of Chinese Medicine;Jiangsu Engineering Research Center for Efficient Delivery System of TCM;Jiangsu Key Laboratory of Pediatric Respiratory Disease, Institute of Pediatrics, Nanjing University of Chinese Medicine;Hunan Yandi Biological Engineering Co., Ltd.;

摘要(Abstract):

采用血清非靶向代谢组学技术研究蛹虫草抗非小细胞肺癌药效作用的潜在机制。以Balb/c裸鼠构建人肺癌荷瘤裸鼠移植瘤模型,比较模型组与顺铂组,冬虫夏草低、中、高剂量组,蛹虫草低、中、高剂量组中移植瘤裸鼠的肿瘤体积、肿瘤质量及抑瘤率,以评价蛹虫草对肺癌生长的影响。收集移植瘤裸鼠血清样本,使用气相色谱-质谱(gas chromatography-mass spectrometry, GC-MS)仪器进行检测,运用SIMCA 13.0软件开展血清代谢轮廓比较,采用MetaboAnalyst 5.0探究相关代谢通路。药效学数据表明,与模型组相比,蛹虫草高剂量组和顺铂组裸鼠的肿瘤体积与肿瘤组织质量显著减小(P<0.05或P<0.01)。代谢组学结果显示,蛹虫草组与模型组裸鼠血清的代谢轮廓具有明显差异,其内源性代谢物含量受不同程度的调整,初步鉴定出42个差异代谢物及7条代谢通路。综上,蛹虫草能够显著抑制人肺癌移植瘤裸鼠肿瘤的生长,其潜在作用机制可能与影响氨酰-tRNA合成,谷氨酰胺和谷氨酸代谢,丙氨酸、天冬氨酸和谷氨酸代谢,乙醛酸和二羧酸代谢,苯丙氨酸、酪氨酸和色氨酸生物合成,精氨酸生物合成及氮代谢等通路密切相关。该研究从代谢物角度阐释了蛹虫草抗非小细胞肺癌的作用机制,为蛹虫草抗肿瘤研究及临床应用奠定基础。
This study investigated the potential mechanism of Cordyceps militaris(CM) against non-small cell lung cancer(NSCLC) based on serum untargeted metabolomics. Specifically, Balb/c nude mice were used to generate the human lung cancer A549 xenograft mouse model. The tumor volume, tumor weight, and tumor inhibition rate in mice in the model, cisplatin, Cordyceps(low-, medium-, and high-dose), and CM(low-, medium-, and high-dose) groups were compared to evaluate the influence of CM on lung cancer. Gas chromatography-mass spectrometry(GC-MS) was used for the analysis of mouse serum, SIMCA 13.0 for the compa-rison of metabolic profiles, and MetaboAnalyst 5.0 for the analysis of metabolic pathways. According to the pharmacodynamic data, the tumor volume and tumor weight of mice in high-dose CM group and cisplatin group decreased as compared with those in the model group(P<0.05 or P<0.01). The results of serum metabolomics showed that the metabolic profiles of the model group were significantly different from those of the high-dose CM group, and the content of endogenous metabolites was adjusted to different degrees. A total of 42 differential metabolites and 7 differential metabolic pathways were identified. In conclusion, CM could significantly inhibit the tumor growth of lung cancer xenograft mice. The mechanism is the likelihood that it influences the aminoacyl-tRNA biosynthesis, the metabolism of D-glutamine and D-glutamate, metabolism of alanine, aspartate, and glutamate, metabolism of glyoxylate and dicarboxylic acid, biosynthesis of phenylalanine, tyrosine, and tryptophan, arginine biosynthesis as well as nitrogen metabolism. This study elucidated the underlying mechanism of CM against NSCLC from the point of metabolites. The results would lay a foundation for the anticancer research and clinical application of CM.

关键词(KeyWords): 蛹虫草;冬虫夏草;肿瘤;非小细胞肺癌;血清代谢组学
Cordyceps militaris;Cordyceps;tumor;non-small cell lung cancer;serum metabolomics

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(82074024,82104688);; 江苏省自然科学基金项目(BK20190800);; 江苏省高等学校自然科学研究项目(19KJB360004);; 南京中医药大学自然科学基金项目(XPT82104688)

作者(Authors): 陆颖颖;黄枭;罗子宸;祁明媛;单进军;张雯;狄留庆;
LU Ying-ying;HUANG Xiao;LUO Zi-chen;QI Ming-yuan;SHAN Jin-jun;ZHANG Wen;DI Liu-qing;School of Pharmacy, Nanjing University of Chinese Medicine;Jiangsu Engineering Research Center for Efficient Delivery System of TCM;Jiangsu Key Laboratory of Pediatric Respiratory Disease, Institute of Pediatrics, Nanjing University of Chinese Medicine;Hunan Yandi Biological Engineering Co., Ltd.;

DOI: 10.19540/j.cnki.cjcmm.20220613.702

参考文献(References):

扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享