中国中药杂志

2008, (20) 2369-2373

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HPLC-MS/MS测定大鼠血浆中的芍药苷及其药动学研究
Determination of paeoniflorin in rat plasma by HPLC-MS/MS and its pharmacokinetics

武洁;姚楠;王大为;
WU Jie,YAO Nan,WANG Da-wei(Jiangsu Provincial Insititute of Traditional Chinese Medicine,Nanjing 210028,China)

摘要(Abstract):

目的:建立HPLC-MS/MS分析方法测定大鼠血浆中的芍药苷含量,并用于研究当归对赤芍主要有效成分芍药苷的药动学影响。方法:质谱检测方式:多反应离子监测,选择监测的离子为m/z450~m/z327(芍药苷)和m/z388~m/z225(栀子苷)。大鼠分别灌胃赤芍煎液和赤芍及当归煎液,芍药苷剂量均为294.78 mg.kg-1。HPLC-MS/MS法测定芍药苷的血药浓度,并计算芍药苷药动学参数。结果:单独服用赤芍煎液时芍药苷的主要药动学参数分别为Cmax(1.55±0.53)mg.L-1,Tmax(0.9±0.3)h,t1/2(1.51±0.63)h,MRT(3.08±0.74)h,AUC0→τ(4.68±0.85)mg.h-1.L-1。同时服用当归煎液和赤芍煎液时芍药苷的Cmax,Tmax,t1/2,MRT,AUC0→τ分别为(0.93±0.42)mg.L-1,(1.5±0.8)h,(3.08±1.79)h,(5.19±1.95)h,(3.36±0.56)mg.h-1.L-1。两组药动学参数中,MRT,Cmax和AUC0→τ具有显著性差异(P<0.05)。结论:当归能显著影响赤芍主要有效成分芍药苷的药动学。
Objective: An HPLC-MS/MS assay was established to determine paeoniflorin in rat plasma and be used to investigate the effect of pharmacokinetics of paeoniflorin when co-administrated Radix Angelicae Sinensis and Radix Paeoniae Rubra.Method: HPLC-MS/MS was performed in the multiple reaction monitoring(MRM) mode using target ions at m/z 450→m/z 327 for paeoniflorin and m/z 388→m/z 225 for jasminoidin.A single dose of Radix Paeoniae Rubra alone and with Radix Angelicae Sinensis was given to rats by ig,the dosage of paeoniflorin was 294.78 mg·kg-1.Concentrations of paeoniflorin in rat plasma were determined by HPLC-MS/MS assay and main pharmacokinetic parameters were estimated.Result: The mainly pharmacokinetic parameters of paeoniflorin when administrated Radix Paeoniae Rubra only were as follows: Cmax(1.55±0.53) mg·L-1,Tmax(0.9±0.3) h,t1/2(1.51±0.63) h,MRT(3.08±0.74) h,AUC0→τ(4.68±0.85) mg·h-1·L-1.When the two drugs administrated together,the corresponding parameters were(0.93±0.42) mg·L-1,(1.5±0.8) h,(3.08±1.79) h,(5.19±1.95) h,(3.36±0.56) mg·h-1·L-1,respectively.The parameters of Cmax,AUC0→τ and MRT were significantly changed(P<0.05).Conclusion: Radix Angelicae Sinensis can significantly affect the pharmacokinetics of paeoniflorin,the principal component of Radix Paeoniae Rubra.

关键词(KeyWords): 芍药苷;HPLC-MS/MS;药动学;当归
paeoniflorin;HPLC-MS/MS;pharmacokinetics;Radix Angelicae Sinensis

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作者(Author): 武洁;姚楠;王大为;
WU Jie,YAO Nan,WANG Da-wei(Jiangsu Provincial Insititute of Traditional Chinese Medicine,Nanjing 210028,China)

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