中国中药杂志

2019, v.44(21) 4720-4727

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UPLC-Qtrap-MS法分析鉴定肉桂水提物给药后大鼠血浆、胆汁、尿液和粪便中的药源性成分
Identification of metabolites in rat plasma,bile,urine and feces after oral administration of Cinnamomi Cortex aqueous extract by UPLC-Qtrap-MS

施梦玲;宋月林;陈金凤;何珊;郭晓宇;屠鹏飞;李军;姜勇;
SHI Meng-ling;SONG Yue-lin;CHEN Jin-feng;HE Shan;GUO Xiao-yu;TU Peng-fei;LI Jun;JIANG Yong;State Key Laboratory of Natural and Biomimetic Drugs,School of Pharmaceutical Sciences,Peking University;Modern Research Center for Traditional Chinese Medicine,School of Chinese Materia Medica,Beijing University of Chinese Medicine;

摘要(Abstract):

研究肉桂水提物主要成分在正常大鼠血浆、胆汁、尿液和粪便中的药源性成分,推测其代谢途径。通过灌胃给予大鼠肉桂水提物后,收集血浆、胆汁、尿液和粪便并进行预处理,采用UPLC-Qtrap-MS技术,综合使用增强型全扫描、母离子扫描、中性丢失扫描以及多离子监测等模式,从复杂生物基质中快速筛选目标成分。通过与对照品比对保留时间、相对分子质量和碎片离子以及分析总结文献报道的肉桂中原型成分的裂解规律,进行代谢产物解析。灌胃给药后,大鼠体内共检测并初步鉴定了5个原型成分和76个可能的代谢产物,包括原花青素类及其代谢产物44个,木脂素类代谢产物29个,二萜类成分3个,其他类成分5个。肉桂水提物中化学成分在大鼠体内的代谢途径主要包括脱糖基化、甲基化、硫酸化和葡萄糖醛酸化反应。该研究有助于阐明肉桂体内吸收代谢机制,为其临床应用以及进一步的开发提供有益参考。
An ultra-performance liquid chromatography hybrid triple quadrupole-linear ion trap mass spectrometry(UPLC-QtrapMS) method was established to identify the metabolites in rat plasma,bile,urine and feces after oral administration of Cinnamomi Cortex(CC) aqueous extract. Several survey experiments,such as enhanced mass spectrum scan(EMS),precursor ion scan(PI),neutral loss scan(NL) and multiple ions monitoring(MIM) were applied to search target components,and two separate enhanced product ion(EPI) scans were triggered via information-dependent acquisition(IDA) method to generate the MS/MS spectra. According to the mass spectrometric data collected from reference standards and reported literature,the structures of metabolites were deduced. A total of76 metabolites and 5 original compounds were tentatively identified in rats after oral administration of CC aqueous extract. Deglycosylation,methylation,sulfonation,and glucuronidation were observed as the primary metabolic pathways for the chemical constituents of CC. These data are able to benefit the clarification of the therapeutic material basis,the clinical usage and further R&D of CC.

关键词(KeyWords): 肉桂;水提物;UPLC-Qtrap-MS;代谢产物;原花青素;木脂素;二萜
Cinnamomi Cortex;aqueous extract;UPLC-Qtrap-MS;metabolites identification;proanthocyanidins;lignans;diterpenoids

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81530097,81222051);; 国家“重大新药创制”科技重大专项(2018ZX09711001-008-003)

作者(Author): 施梦玲;宋月林;陈金凤;何珊;郭晓宇;屠鹏飞;李军;姜勇;
SHI Meng-ling;SONG Yue-lin;CHEN Jin-feng;HE Shan;GUO Xiao-yu;TU Peng-fei;LI Jun;JIANG Yong;State Key Laboratory of Natural and Biomimetic Drugs,School of Pharmaceutical Sciences,Peking University;Modern Research Center for Traditional Chinese Medicine,School of Chinese Materia Medica,Beijing University of Chinese Medicine;

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DOI: 10.19540/j.cnki.cjcmm.20190807.202

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