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正交试验法对大黄总蒽醌衍生物提取条件的优选
Selection of the Extraction Conditions for Total Anthraquinone Derivatives in Rhubarb by Using Orthogonal Experiment Design

丁安伟,黄敏,孔令东,吴皓
Ding Anwei Huang Min Kong Lingdong and Wu Hao (Department of Traditional Pharmacology,Nanjing College of Traditional Chinese Medicine,Nanjing)

摘要(Abstract):

对大黄中总蒽醌衍生物的提取方法做了某些改进,并使用正交试验法对提取条件进行优选.结果表明,以加入混合酸[冰HAc-25%HCl(10∶2)]20ml,水解2小时后氯仿提取较好。
The method of extraction for total anthraquinone derivatives in Rhubarb was improved in someaspects,and the extraction conditions were selected by using orthogonal experiment design.Theresult shows that a better way to extract the total anthraquinone derivatives is to add 20ml ofa mixed acid(glacial acetic acid:25 percent hydrochloric acid solution=10:2)to the powder ofRhubarb,let the latter hydrolyze for 2 hours and then proceed to extracting with chloroform.

关键词(KeyWords): 大黄;蒽醌;正交试验
Rhubarb;anthraquinone;orthogonal experiment design

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作者(Author): 丁安伟,黄敏,孔令东,吴皓
Ding Anwei Huang Min Kong Lingdong and Wu Hao (Department of Traditional Pharmacology,Nanjing College of Traditional Chinese Medicine,Nanjing)

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