中国中药杂志

2015, v.40(16) 3187-3193

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蒲黄-五灵脂配伍前后效应成分变化及其抗血小板聚集和抗凝血酶活性评价
Study on antiplatelet and antithrombin activitives and effective components variation of Puhuang-Wulingzhi before and after compatibility

宿树兰;薛萍;欧阳臻;周卫;段金廒;
SU Shu-lan;XUE Ping;OUYANG Zhen;ZHOU Wei;DUAN Jin-ao;Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization,National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine,Jiangsu Key Laboratory for Traditional Chinese Medicine Formulae Research,Nanjing University of Chinese Medicine;School of Pharmacy,Jiangsu University;

摘要(Abstract):

为了探讨蒲黄-五灵脂药对配伍的科学性与合理性,对蒲黄-五灵脂配伍前后效应成分的溶出变化进行分析,并进一步评价其与抗血小板聚集和抗凝血酶活性的相关性。采用超高效液相色谱飞行时间串联质谱仪(UPLC-QTOF-MS-Markerlynx)联用技术分析蒲黄-五灵脂配伍前后效应成分群的溶出变化;采用体外实验评价蒲黄-五灵脂配伍前后对ADP诱导的家兔血小板聚集及抗凝血酶活性。结果表明,蒲黄-五灵脂分煎混合液与配伍合煎液总离子流色谱峰存在明显不同,鉴定出5个相对含量具有显著差异的化合物,分别为香蒲新苷、柚皮素、异鼠李素-3-O-芸香糖苷、槲皮素-3-O-新橙皮糖苷、山柰酚-3-O-新橙皮糖苷。活性评价结果表明,蒲黄-五灵脂合煎液抗血小板聚集和延长凝血酶时间活性优于蒲黄、五灵脂单煎液活性。研究结果为蒲黄-五灵脂两药配伍后效应物质基础及效应机制的阐明提供了一定的科学依据。
The changes of bioactive constituents were analyzed for Puhuang-Wulingzhi before and after compatibility and the antiplatelet and antithrombin activitives were evaluated in order to elucidate the scientific and reasonable of Puhuang-Wulingzhi compatibility. UPLC-QTOF-MA-Markerlynx,principal component analysis( PCA) and orthogonal partial least-squares discriminant analysis were used for data analysis and tracking changes of chemical composition during the decocting process. In vitro platelet aggregation induced by ADP,thrombin time( TT) and prothrombin time( PT) were investigated for Puhuang-Wulingzhi before and after compatibility. The results showed that significant differences were found between the mixed decoction and codecoction of Wulingzhi and Puhuang. Five compounds changed obviously were identified as typhaneoside,naringenin,isorhamnetin-3-O-ruinoside,quercetin-3-O-neohesperidoside,kaempferol-3-O-neohesperidoside. The codecoction,comparing with the single decoction,was more significant in antiplatelet aggregation and could prolong thrombin time. In the same crude drug dose,the thrombin time( TT) elongation were greater. These data could provide references for elucidation of bioactive components for this herb pair.

关键词(KeyWords): 蒲黄;五灵脂;药对;黄酮类;有机酸类
Puhuang;Wulingzhi;herbal pair;flavonoids;organic acid

Abstract:

Keywords:

基金项目(Foundation): 江苏省优势学科建设工程中药学一级学科建设项目(YS-2014);; 江苏省高校自然科学重大基础研究项目(06KJA36022,11KJA360002);; 江苏省方剂高技术研究重点实验室建设项目(BM2010576)

作者(Author): 宿树兰;薛萍;欧阳臻;周卫;段金廒;
SU Shu-lan;XUE Ping;OUYANG Zhen;ZHOU Wei;DUAN Jin-ao;Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization,National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine,Jiangsu Key Laboratory for Traditional Chinese Medicine Formulae Research,Nanjing University of Chinese Medicine;School of Pharmacy,Jiangsu University;

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