中国中药杂志

2015, v.40(14) 2743-2747

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基于代谢组学技术研究参附配伍对大鼠心脏毒性的影响
Cardiotoxicity study of Shenfu compatibility in rats based on metabonomics

何家乐;赵佳伟;马增春;梁乾德;王宇光;谭洪玲;肖成荣;汤响林;高月;
HE Jia-le;ZHAO Jia-wei;MA Zeng-chun;LIANG Qian-de;WANG Yu-guang;TAN Hong-ling;XIAO Cheng-rong;TANG Xiang-lin;GAO Yue;Graduate School of Anhui Medical University;Institute of Radiation Medicine Sciences,Academy of Military Medical Sciences;

摘要(Abstract):

运用UPLC-Q-TOF/MS技术研究人参与附子配伍对大鼠血清中内源性标记物的影响,旨在发现人参对附子减毒作用的内源性标记物及其分子机制。连续7 d给予健康雄性Wistar大鼠不同配伍的参附水煎液20 g·kg-1,收集血清,通过主成分分析和偏最小二乘判别法分析参附配伍对内源性代谢物的影响。参附合并组中,谷胱甘肽、磷脂酰胆碱、柠檬酸含量下降,同时抗坏血酸、尿酸、D-半乳糖、色氨酸、L-苯丙氨酸的含量上升。说明参附合煎液中附子对心脏产生了毒性影响。参附合煎组中与空白组对比表现出了类似或者稍弱的趋势,但是大部分并不具有统计学意义。对比参附合煎组与合并组,可以得出参附配伍减毒的科学性。结果可见,人参与附子配伍可以减轻附子的心脏毒性,柠檬酸、谷胱甘肽、磷脂酰胆碱、尿酸可作为附子造成心脏毒性的潜在标记物。
To research the effect of Ginseng Radix et Rhizoma and Aconiti Lateralis Radix Praeparata compatibility on cardiac toxicity in rats by UPLC-Q-TOF / MS,and explore the endogenous markers and molecule mechanism. Different compatibility of Shenfu decoction were given to male Wistar rats at dosage of 20 g·kg-1for 7 days,collected the serum,and analyze the endogenous metabolites effected by Shenfu formulation by principal component analysis and partial least-squares analysis. Results showed that content of glutathione,phosphatidylcholine and citric acid decreased in mixed-decoction group,while ascorbic acid,uric acid,D-galactose,tryptophan,L-phenylalanine increased. The results showed cardiac toxicity of Aconiti Lateralis Radix Praeparata in Shenfu mixed-decoction.Shenfu co-decoction group showed a similar or weaker trend compared with control group,but most of them do not have a statistically significant. The results indicated the scientific basis of Shenfu compatibility by comparison of co-decoction group with mixed-decoction group. Shenfu compatibility can reduce cardiac toxicity induced by Aconiti Lateralis Radix Praeparata,and citric acid,glutathione,phosphatidyl choline,uric acid might be regarded as potential markers of cardiotoxicity.

关键词(KeyWords): 人参;附子;增效减毒;内源性标示物;代谢组学
Ginseng Radix et Rhizoma;Aconiti Lateralis Radix Praeparata;synergism and attenuation;endogenous makers;metabolomics

Abstract:

Keywords:

基金项目(Foundation): 国家重点基础研究发展计划(973)项目(2011CB505304,2012CB518402);; 国家自然科学基金项目(81274127)

作者(Authors): 何家乐;赵佳伟;马增春;梁乾德;王宇光;谭洪玲;肖成荣;汤响林;高月;
HE Jia-le;ZHAO Jia-wei;MA Zeng-chun;LIANG Qian-de;WANG Yu-guang;TAN Hong-ling;XIAO Cheng-rong;TANG Xiang-lin;GAO Yue;Graduate School of Anhui Medical University;Institute of Radiation Medicine Sciences,Academy of Military Medical Sciences;

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