中国中药杂志

2009, v.34(02) 230-233

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

麦冬多糖在正常和缺血大鼠心脏分布的比较研究
Comparison of distribution of Ophiopogon Japonicas polysaccharide in hearts of normal rats and myocardial ischemic rats

林晓;卢智玲;徐德生;冯怡;
LIN Xiao~1 LU Zhiling~(1,2) XU Desheng~3 FENG Yi~(1*) (1. Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China; 2. Hangzhou drug control institute, Hangzhou 310017, China; 3. Affiliated Shuguang Hospital of Shanghai University of Traditional Chinese Medicine, Shanghai 200021, China)

摘要(Abstract):

目的:研究麦冬多糖在正常和缺血大鼠心脏分布差异程度,并分析差异原因,为其作用机制的确定提供进一步的理论依据。方法:以50 mg·kg~(-1)剂量静脉注射给予正常组和心肌缺血组大鼠麦冬多糖,分别于给药后的5,10,30,60 min时间点取心脏,用HPGPC-RF测定多糖组织分布情况。结果:心肌缺血组除30 min时间点外,其余各个时间点均比正常组的分布明显增多,且经SPSS软件统计有显著性差异。结论:缺血所导致的增强的透过和滞留效应(即EPR效应,the enhanced permeability and retention effect)要强于血流减少对麦冬多糖分布所带来的负面影响,有利于麦冬多糖靶向心肌缺血区而发挥其药理活性。
Objective:To investigate the distribution of Ophiopogon Japonicas polysaccharide (OJP) in hearts of normal rats and myocardial ischemic rats,and obtain the further evidences for determining the mechanism of its anti-myocardial ischemia. Method:Hearts of normal and myocardial ischemic groups were obtained at 5,10,30 and 60 min after once intravenous administration of OJP with the dose of 50 mg/kg.The concentration of OJP in samples was subsequently determined by HPGPC with a fluorescence detector.Result:The distribution of OJP in hearts of myocardial ischemic rats was much more than that in normal groups at all the sampling times.Moreover,the differences were proved to be significant by SPSS software with the exception of the situation in the 30 min sampling time.Conclusion:The enhanced permeability and retention effect caused by ischemia can overcome the negative effect of the reduction of blood flow on the distribution of OJP from blood into myocardial ischemic zones and actually help OJP target myocardial ischemic zones where it can exert its pharmacological effect directly.

关键词(KeyWords): 麦冬;多糖;EPR效应;心肌缺血
Ophiopogon Japonicas;polysaccharide;enhanced permeability and retention effect;myocardial ischemia

Abstract:

Keywords:

基金项目(Foundation): 上海市青年科技启明星计划(07QA14050);; 国家中医药管理局中医药科学技术专项(06-07ZQ04)

作者(Author): 林晓;卢智玲;徐德生;冯怡;
LIN Xiao~1 LU Zhiling~(1,2) XU Desheng~3 FENG Yi~(1*) (1. Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China; 2. Hangzhou drug control institute, Hangzhou 310017, China; 3. Affiliated Shuguang Hospital of Shanghai University of Traditional Chinese Medicine, Shanghai 200021, China)

Email:

DOI:

扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享