中国中药杂志

2013, v.38(06) 884-888

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玉红膏重复给药大鼠体内汞的吸收及蓄积研究
Study on absorption and accumulation of mercury in rats by repeated administration of Yuhong ointment

邱恒;孙新民;黄雯;胡小靖;王旗;牟稷征;王丽霞;
QIU Heng1,SUN Xin-min1,HUANG Wen1,HU Xiao-jing1,WANG Qi1*,MOU Ji-zheng 2,WANG Li-xia2(1.Department of Toxicology,School of Public Health,Peking University,Beijing 100191,China; 2.Guang′anmen Hospital,China Academy of Chinese Medical Sciences,Beijing 100053,China)

摘要(Abstract):

目的:通过经皮重复给予大鼠不同浓度含轻粉玉红膏,考察体内汞的吸收及蓄积情况,为临床安全用药提供科学依据。方法:将100只SD大鼠随机分成5组:对照组、玉红膏组、2倍浓度玉红膏组、4倍浓度玉红膏组和1.6%轻粉组。于破损皮肤重复给药(剂量为0.04 g·cm-2)2周、4周和停药4周时,分别测定大鼠血、尿中汞含量和心、肝、脑、肾组织中汞的蓄积量。结果:连续给药4周时,玉红膏组大鼠血中汞含量与对照组比较无明显差异,其他各实验组随着给药时间的延长和给药剂量的增高,吸收入血的汞含量和尿中汞的排泄量均明显增高。给药4周时,实验组各组织汞含量均升高,其中肾最高,脑最低。停药4周时,各实验组大鼠血、尿中汞含量均恢复正常,各组织汞含量明显下降。结论:玉红膏经破损皮肤给予大鼠4周,吸收入血的汞量甚微,体内汞的蓄积主要在肾脏,汞主要从尿液排泄。提示临床长期使用玉红膏应监测患者体内汞含量和肾功能指标。
Objective: To study in vivo mercury absorption and accumulation through repeated transdermal administration of Yuhong ointment containing calomel,in order to provide scientific evidences for clinical safe medication.Method: A total of 100 SD rats were randomly classified into five groups: the control group,the Yuhong ointment group,the double-concentration Yuhong Ointment group,the quadruple-concentration Yuhong ointment group and the 1.6% calomel group.The rats were treated with the dosage of 0.04 g·cm-2 by repeated transdermal administration for 2,4 weeks.After the drug discontinuance for 4 weeks,the levels of mercury in blood,urine,and tissues of heart,liver,brain and kidney were determined,respectively.Result: Compared with the control group,the blood mercury level of the Yuhong ointment group show no obvious change after treatment for 4 weeks.However,the levels of mercury in blood and urine of other experimental groups increased significantly with time and the increase in dosage,and so did the level of mercury in major organ.At 4 weeks,all experimental groups showed increase in the content of mercury,and kidneys displayed the highest level,whereas brain displayed the lowest level.After the drug discontinuance for 4 weeks,the mercury level in blood and urine of every dose group recovered to normal,with significant decline in the content of mercury in each organ.Conclusion: After transdermal administration in rats for 4 weeks,there was no obvious absorption of mercury in blood.Mercury was mainly accumulated in kidneys and excreted through urine.The results suggest that the patients′ mercury content and kidney function indexes need to be monitored in long-term clinical use of Yuhong ointment.

关键词(KeyWords): 玉红膏;汞;经皮给药;大鼠
Yuhong ointment;mercury;transdermal administration;rat

Abstract:

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基金项目(Foundation): 国家“十一五”科技支撑计划项目(2008BAI53B084)

作者(Author): 邱恒;孙新民;黄雯;胡小靖;王旗;牟稷征;王丽霞;
QIU Heng1,SUN Xin-min1,HUANG Wen1,HU Xiao-jing1,WANG Qi1*,MOU Ji-zheng 2,WANG Li-xia2(1.Department of Toxicology,School of Public Health,Peking University,Beijing 100191,China; 2.Guang′anmen Hospital,China Academy of Chinese Medical Sciences,Beijing 100053,China)

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