薛东芳;邹宗尧;陈彪;王燕枝;吴昊;叶小利;李学刚;

• 1:西南大学生命科学学院
• 2:重庆药物过程与质量控制工程技术研究中心
• 3:西南大学药学院

摘要(Abstract)：

为探索黄连总生物碱及小檗碱对嗜水气单胞菌的抑菌活性及作用机制,测定了黄连总生物碱和小檗碱对嗜水气单胞菌的最小抑菌浓度、膜通透性、膜流动性、膜蛋白构象及毒力因子的影响。结果显示,黄连总生物碱和小檗碱对嗜水气单胞菌均有抑菌活性,其最小抑菌浓度分别为62.5,125 mg·L-1。黄连总生物碱和小檗碱能增加膜脂流动性,改变膜蛋白构象,并使菌体膜通透性分别增加24.52%,19.66%。此外,黄连总生物碱和小檗碱显著性地抑制菌体外毒素的溶血活性及aer A和hly A mRNA的表达(P<0.05,P<0.01),同时降低嗜水气单胞菌内毒素的分泌量和Lpx C mRNA的表达(P<0.05,P<0.01)。结果表明,黄连总生物碱和小檗碱对嗜水气单胞菌的抑菌作用可能与细胞膜损伤有关,通过改变膜脂流动性和膜蛋白构象使菌体膜通透性增加从而抑制了细菌生长;同时降低了细菌的毒力因子的分泌,使得菌体的致病性降低。

关键词(KeyWords)： 黄连生物碱;嗜水气单胞菌;抑菌机制

Abstract:

Keywords:

基金项目(Foundation): 国家“十二五”科技支撑计划项目(2011BAI13B02-1);; 重庆市教委重点项目(KJZH14203);; 教育部博士点基金(20130182110023);; 重庆市百名高端工程技术人才培养计划(2013-2016)

作者(Author): 薛东芳;邹宗尧;陈彪;王燕枝;吴昊;叶小利;李学刚;

Email:

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