中国中药杂志

2010, v.35(16) 2091-2094

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草豆蔻药材质量控制方法研究
Qualitative and quantitative methods for Alpiniae Katsumadai Semen

李元圆;侴桂新;杨莉;王峥涛;
LI Yuanyuan1,2,3,CHOU Guixin2,3,YANG Li2,WANG Zhengtao2,3 (1.Zhejiang Medical College,Hangzhou 310053,China;2.MOE Key Laboratory of Standardization of Chinese Medicines,Shanghai Universtiy of Traditional Chinese Medicine,Shanghai 201203,China;3.Shanghai Research and Development Centre for Standardization of Chinese Medicines,Shanghai 201203,China)

摘要(Abstract):

目的:建立草豆蔻药材的定性鉴别和定量检测方法。方法:运用薄层色谱法和高效液相色谱法对草豆蔻药材中山姜素(alpinetin)、乔松素(pinocembrin)、小豆蔻明(cardamonin)和桤木酮(alnustone)进行定性鉴别和含量测定。以UltimateXB-C18色谱柱(4.6 mm×250 mm,5μm)为固定相,甲醇-水为流动相,梯度洗脱,检测波长300 nm,柱温30℃。结果:草豆蔻药材中山姜素、乔松素、小豆蔻明和桤木酮的薄层色谱鉴别特征明显,专属性强;4个指标成分在进样量25.5~509(r=0.9999),24.9~498(r=0.999 9),26.1~521(r=0.999 9),50.2~1003 ng(r=0.999 9)与峰面积线性关系良好;平均加样回收率(n=9)分别为97.95%,97.36%,97.50%,98.02%,RSD均小于1.9%。结论:通过对9批不同来源的草豆蔻药材的薄层鉴别和含量测定,证明该方法简单灵敏,专属性强,准确可靠,重复性好,为全面评价草豆蔻药材质量提供了参考。
The qualitative and quantitative methods for the quality evaluation of Alpiniae Katsumadai Semen were established.Alpinetin,pinocembrin,cardamonin and alnustone in the sample were identified by TLC.The contents of them were determined by HPLC.The separation was performed on a Ultimate XB-C18 column(4.6 mm×250 mm,5 μm) at 30 ℃ using a gradient elution consisting of mobile phase A(water) and mobile phase B(MeOH).The detection wavelength was 300 nm.The TLC method was suitable for the identification of alpinetin,pinocembrin,cardamonin and alnustone.The linear ranges of the four reference compounds were 25.5-509(r=0.999 9),24.9-498(r=0.999 9),26.1-521(r=0.999 9),50.2-1 003 ng(r=0.999 9),respectively.The average recoveries(n=9)of the four components were 97.95%,97.36%,97.50%,98.02%,RSD<1.9%.Nine samples were analyzed by the established methods.The results indicate that,the methods are simple,accurate,sensitive and reliable for quality evaluation of Alpiniae Katsumadai Semen.

关键词(KeyWords): 草豆蔻;山姜素;乔松素;小豆蔻明;桤木酮;薄层色谱法;质量标准
Alpiniae Katsumadai Semen;alpinetin;pinocembrin;cardamonin;alnustone;TLC;quality standard

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基金项目(Foundation): 《中国药典》2010版一部标准研究(YD-013)

作者(Author): 李元圆;侴桂新;杨莉;王峥涛;
LI Yuanyuan1,2,3,CHOU Guixin2,3,YANG Li2,WANG Zhengtao2,3 (1.Zhejiang Medical College,Hangzhou 310053,China;2.MOE Key Laboratory of Standardization of Chinese Medicines,Shanghai Universtiy of Traditional Chinese Medicine,Shanghai 201203,China;3.Shanghai Research and Development Centre for Standardization of Chinese Medicines,Shanghai 201203,China)

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