中国中药杂志

2016, v.41(08) 1493-1497

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芩百清肺浓缩丸对肺炎支原体感染大鼠肺泡Ⅱ型上皮细胞修复作用的研究
Repair effect of Qinbai Qingfei concentrated pellets to AEC-Ⅱ of rats infected by Mycoplasmal pneumonia

姚琳;张俊威;王博;隋美娇;王伟明;
YAO Lin;ZHANG Jun-wei;WANG Bo;SUI Mei-jiao;WANG Wei-ming;Heilongjiang Academy of Chinese Medical Sciences;

摘要(Abstract):

通过对转化生长因子-β(TGF-β)和表面活性相关蛋白A(SP-A)给药前后的表达变化及分布,探讨芩百清肺浓缩丸对肺炎支原体(MP)感染大鼠肺泡Ⅱ型上皮细胞的修复作用机制。取60只Wistar大鼠,雌雄各半,体重80~100 g,随机分为6组,每组10只,分别为空白组,模型组,阳性对照组,芩百清肺浓缩丸高、中、低剂量组,采用滴鼻法造模;给药10 d后收集大鼠肺泡灌洗液、血清、左肺及右肺组织中叶,采用免疫组化法检测肺组织中TGF-β及SP-A的蛋白表达及分布;实时荧光定量PCR法测定肺组织中TGF-β,SP-A mRNA的表达;酶联免疫法测定血清及肺泡灌洗液(BALF)中SP-A的含量。通过数据分析可知,芩百清肺浓缩丸能够下调肺组织中TGF-β的表达,增加SP-A的表达,其中TGF-β主要分布于肺间质,SP-A主要分布于AEC-Ⅱ及部分肺泡巨噬细胞内;同时,能够降低血清中SP-A含量,增加BALF中SP-A含量。通过以上结果可知,芩百清肺浓缩丸通过降低TGF-β的表达抑制AEC-Ⅱ发生上皮间质转化,并增加SP-A的表达,恢复肺的正常形态与功能;同时,对MP引起的肺组织毛细血管损伤具有一定的修复作用。
To discuss the repair mechanism of Qinbai Qingfei concentrated pellets to AEC-Ⅱ of rats infected by mycoplasma through the observation of the changes and distribution of TGF-β and SP-A in lungs,totally 60 Wistar rats that weighing 80-100 g were collected,with male and female in half. The rats were divided into six groups randomly,with 10 rats each group,namely blank group,model group,positive group and Qinbai Qingfei concentrated pellets high,middle and low dose groups. Rats were infected through nasal intubation drip of MP. After 10 days of administration,serum and bronchoalveolar lavage fluid( BALF) were collected to detect the concentration of surface activity related protein A( SP-A) by ELISA,left pulmonary tissues of rats were collected to observe the expression and distribution of transforming growth factor-beta( TGF-β) and SP-A by immunohistochemistry,and right pulmonary tissues were taken to detect TGF-β and SP-A mRNA expression level by real-time quantitative PCR( RT-PCR). Qinbai Qingfei concentrated pellets can reduce the expression of TGF-β and increase the expression of SP-A in the lung tissues of rats infected by mycoplasma. Specifically,TGF-β was mainly distributed among the lung interstitium,while SPAs were mainly distributed in AEC-II and parts of alveolar macrophage. The level of SP-A was reduced in serum and increased in BALF in rats in Qinbai Qingfei concentrated pellets groups. It was proved that Qinbai Qingfei concentrated pellets can restore the normal morphology and function of the lung by reducing the content of TGF-β to inhibit epithelial-mesenchymal transition( EMT) of alveolar type II epithelial cells and increasing the expression of SP-A.Qinbai Qingfei concentrated pellets have the repairing ability to capillary vessel damage caused by MP in lung tissues of rats.

关键词(KeyWords): 肺炎支原体;芩百清肺浓缩丸;肺泡Ⅱ型上皮细胞;转化生长因子-β;表面活性相关蛋白A
Mycoplasma Pneumoniae;Qinbai Qingfei concentrated pellets;AEC-Ⅱ;TGF-β;SP-A

Abstract:

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基金项目(Foundation): 国家“重大新药创制”科技重大专项(2010ZX09101-104);; 国家自然科学基金项目(81374045)

作者(Author): 姚琳;张俊威;王博;隋美娇;王伟明;
YAO Lin;ZHANG Jun-wei;WANG Bo;SUI Mei-jiao;WANG Wei-ming;Heilongjiang Academy of Chinese Medical Sciences;

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