中国中药杂志

2011, v.36(06) 694-697

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赤芍和白芍抗炎作用的UPLC-MS代谢组学初步研究
Metabolomics study of anti-inflammatory action of Radix Paeoniae Rubra and Radix Paeoniae Alba by ultraperformance liquid chromatography-mass spectrometry

杨琪伟;杨莉;熊爱珍;鲁岚;王瑞;王峥涛;
YANG Qiwei1,YANG Li1,2,XIONG Aizhen1,2,LU Lan1,WANG Rui1,2*,WANG Zhengtao1,2(1.Shanghai University of Traditional Chinese Medicines,Shanghai 201203,China; 2.Shanghai R&D Center for Standardization of Traditional Chinese Medicines,Shanghai 201203,China)

摘要(Abstract):

目的:通过比较赤芍和白芍对角叉菜胶致大鼠足趾肿胀的影响及内源性代谢物分析,探讨赤芍和白芍抗炎作用的异同。方法:建立角叉菜胶致大鼠足趾肿胀动物模型,观察赤芍和白芍的抗炎作用。基于超高效液相色谱-电喷雾质谱(UPLC-MS)比较赤芍和白芍给药后大鼠血清代谢物指纹图谱,采用偏最小二乘判别分析法研究给药组与正常组之间的代谢物组差异,寻找可能的生物标识物。结果:赤芍和白芍提取物在0.5~1 h对大鼠角叉菜胶致足趾肿胀均有较好的抑制作用,在2~3 h仅赤芍提取物有较好的抑制作用。前列腺素F2α,前列腺素E3,白细胞三烯A4,前列腺素E2,谷胱甘肽等内源性生物标识物对于表征赤芍、白芍的抗炎作用具有重要的作用,且发现赤芍和白芍均可影响机体内前列腺素F2α和前列腺素E3的水平,但赤芍和白芍给药组间有显著性差异,提示二者发挥抗炎作用机制有一定差异。结论:代谢组学初步研究结果与传统药理试验结果有相关性,为进一步深入探讨赤芍和白芍的作用机制异同奠定基础。
Objective: To compare the anti-inflammatory effect of Radix Paeoniae Rubra and Radix Paeoniae Alba by animal experiment and metabolimic analysis.Method: To estabolish the rats model of toes swelling caused by carrageenan,study the anti-inflammatory effect of Radix Paeoniae Rubra and Radix Paeoniae Alba.The serum samples were analyzed by ultraperformance liquid chromatography-mass spectrometry(UPLC-MS),to find out the potential identification biomarker by PLS-DA.Result: Both of the extracts of Radix Paeoniae Rubra and Radix Paeoniae Alba have good effects of inhibition to swelling caused by carrageenan in 0.5-1 h,and the extract of Radix Paeoniae Rubra also show significant inhibition in 2-3 h.Glutathione(GSH),γ-aminobutyric acid(GABA),prostaglandin F2α(PGF2α),prostaglandinE3(PGE3),leukotrieneA4(LTA4),prostaglandinE2(PGE2)are proven to be siginificant expressed biomarkers.Radix Paeoniae Rubra and Radix Paeoniae Alba may have great influence on PGF2α and PGE3.There was also significant difference between the effects of Radix Paeoniae Rubra and Radix Paeoniae Alba,which suggested the difference of anti-inflammatory between the two herbs.Conclusion: The results of metabolomics are related with the results of classic pharmaco-experiment,which is helpful for the further research of the mechanism of action of Radix Paeoniae Rubra and Radix Paeoniae Alba.

关键词(KeyWords): 代谢组学;UPLC-MS;赤芍;白芍;抗炎
metabonomics;UPLC-MS;Radix Paeoniae Rubra;Radix Paeoniae Alba;anti-inflammatory

Abstract:

Keywords:

基金项目(Foundation): 国家“重大新药创制”科技重大专项(2009ZX09502-021,2009ZX09308-003);; 上海市自然科学基金项目(08ZR1419100);; 中医药行业科研专项(200707009)

作者(Author): 杨琪伟;杨莉;熊爱珍;鲁岚;王瑞;王峥涛;
YANG Qiwei1,YANG Li1,2,XIONG Aizhen1,2,LU Lan1,WANG Rui1,2*,WANG Zhengtao1,2(1.Shanghai University of Traditional Chinese Medicines,Shanghai 201203,China; 2.Shanghai R&D Center for Standardization of Traditional Chinese Medicines,Shanghai 201203,China)

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