中国中药杂志

2017, v.42(13) 2558-2563

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二仙汤对去卵巢骨质疏松大鼠股骨蛋白质组的影响
Effect of Er-xian decoction on femur proteomics in ovariectomized osteoporosis rats

刘志文;刘波;吴琪;陈银芳;徐彭;谢慧慧;王莹;杨硕;
LIU Zhi-wen;LIU Bo;WU Qi;CHEN Yin-fang;XU Peng;XIE Hui-hui;WANG Ying;YANG Shuo;College of Pharmacy,Jiangxi University of Chinese Medicine;

摘要(Abstract):

该实验为探究二仙汤防治骨质疏松的作用及对去卵巢骨质疏松模型大鼠股骨蛋白质组的影响。将雌性SD大鼠按体重随机分为4组,即假手术组、模型组、阿仑膦酸钠组(阿仑膦酸钠,1 mg·kg(-1))、二仙汤组(生药量8 g·kg(-1))、二仙汤组(生药量8 g·kg(-1)),其中假手术组和模型组灌服生理盐水,阿仑膦酸钠组按1 mg·kg(-1)),其中假手术组和模型组灌服生理盐水,阿仑膦酸钠组按1 mg·kg(-1)剂量灌服阿仑膦酸钠,二仙汤组按生药量8 g·kg(-1)剂量灌服阿仑膦酸钠,二仙汤组按生药量8 g·kg(-1)灌服二仙汤,每日1次,连续给药90 d后检测股骨骨密度,同时应用Nano-LC-LTQ-Orbitrap系统检测股骨骨蛋白,Protein Discovery软件进行蛋白质鉴定,SIEVE软件对蛋白进行相对定量分析。实验结果显示,二仙汤能显著提高去卵巢大鼠股骨骨密度。二仙汤组与模型组相比较共发现41个与假手术组趋势一致的差异蛋白,主要包括生物氧化相关蛋白、信号转导通路相关蛋白、脂肪酸代谢相关蛋白、细胞骨架相关蛋白、能量代谢相关蛋白、糖代谢相关蛋白等。二仙汤能防治骨质疏松症,碳酸酐酶2、整合素β1等相关差异蛋白质可能是其作用靶点。
This experiment was mainly aimed to investigate the effect of Er-xian decoction on osteoporosis and the femur proteomics in ovariectomized rats with osteoporosis. The female SD rats were randomly divided into sham operation group,model group,Alendronate group(1 mg·kg(-1)灌服二仙汤,每日1次,连续给药90 d后检测股骨骨密度,同时应用Nano-LC-LTQ-Orbitrap系统检测股骨骨蛋白,Protein Discovery软件进行蛋白质鉴定,SIEVE软件对蛋白进行相对定量分析。实验结果显示,二仙汤能显著提高去卵巢大鼠股骨骨密度。二仙汤组与模型组相比较共发现41个与假手术组趋势一致的差异蛋白,主要包括生物氧化相关蛋白、信号转导通路相关蛋白、脂肪酸代谢相关蛋白、细胞骨架相关蛋白、能量代谢相关蛋白、糖代谢相关蛋白等。二仙汤能防治骨质疏松症,碳酸酐酶2、整合素β1等相关差异蛋白质可能是其作用靶点。
This experiment was mainly aimed to investigate the effect of Er-xian decoction on osteoporosis and the femur proteomics in ovariectomized rats with osteoporosis. The female SD rats were randomly divided into sham operation group,model group,Alendronate group(1 mg·kg(-1)),Er-xian decoction group(in dose of 8 g·kg(-1)),Er-xian decoction group(in dose of 8 g·kg(-1)) according to their weight. The rats in sham operation group and model group were gavaged with normal saline; the rats in Alendronate group were gavaged with the Alendronate at the dose of 1 mg·kg(-1)) according to their weight. The rats in sham operation group and model group were gavaged with normal saline; the rats in Alendronate group were gavaged with the Alendronate at the dose of 1 mg·kg(-1)and the rats in Er-xian decoction group were gavaged with Er-xian decoction at the dose of 8 g·kg(-1)and the rats in Er-xian decoction group were gavaged with Er-xian decoction at the dose of 8 g·kg(-1),once a day for continuous 90 days. Then the femoral bone mineral density( BMD) was detected. The femoral bone proteins were detected by Nano LCLTQ-Orbitrap system,identified by Protein Discovery software,and the intensity of differentially expressed proteins were quantitated by SIEVE software. The results showed that Er-xian decoction could significantly improve femoral BMD in ovariectomized rats. As compared with model group,41 differentially expressed proteins whose variation trend was consistent with the sham operation group,were found in Er-xian decoction group,mainly including biological oxidation related protein,signal transduction pathway related protein,proteins involved in aliphatic acid metabolism,cytoskeleton related protein,proteins involved in energy metabolism,and proteins involved in glucose metabolism etc. The osteoporosis could be prevented and cured by Er-xian decoction. The differentially expressed proteins such as carbonic anhydrase 2 and integrin β1 may be the action targets for Er-xian decoction.

关键词(KeyWords): 二仙汤;去卵巢骨质疏松;骨密度;蛋白质组学
Er-xian decoction;ovariectomized osteoporosis;bone mineral density;proteomics

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81560723);; 江西省自然科学基金项目(20151BAB205072);; 江西省卫生和计划生育委员会科技项目(2015B027)

作者(Authors): 刘志文;刘波;吴琪;陈银芳;徐彭;谢慧慧;王莹;杨硕;
LIU Zhi-wen;LIU Bo;WU Qi;CHEN Yin-fang;XU Peng;XIE Hui-hui;WANG Ying;YANG Shuo;College of Pharmacy,Jiangxi University of Chinese Medicine;

DOI: 10.19540/j.cnki.cjcmm.20170609.008

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