中国中药杂志

2005, (18) 1438-1440

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地黄清蒸不同时间5-羟甲基糠醛含量变化研究
Studies on the changes of 5-hydroxymethyl furfuraldehyde content in Radix Rehmanniae steamed for different time

李军,张丽萍,刘伟,张振凌,刘丽娟
LI Jun, ZHANG Li-ping, LIU Wei, ZHANG Zhen-ling, LIU Li-juan(Henan College of Traditional Chinese Medicine, Zhengzhou 450008, China)

摘要(Abstract):

目的:考察地黄清蒸不同时间5-羟甲基糠醛含量的变化规律。方法:采用高效液相色谱法,C18色谱柱(6mm×200mm,5μm),流动相甲醇-水(15∶85),流速1mL.min-1,柱温24℃,检测波长284nm。结果:5-羟甲基糠醛在0.01~0.08μg呈良好线性关系,r=0.9999(n=5),平均回收率100.2%,RSD2.7%(n=5)。结论:地黄在蒸制过程中,5-羟甲基糠醛的含量在一定时间范围内随着时间的延长而增加。
Objective:To find the changing regularity of 5-Hydroxymethyl furfuraldehyde (5-HMF) content in Radix Rehmanniae steamed for different time. Method: HPLC method was used, with C_(18) column (6 mm×200 mm,5 μm), a mixture of methanol-water (15∶85) as the mobile phase. The flow rate was 1.0 mL·min~(-1). The UV detection was setat 284 nm and the column temperature was set at 24 ℃. Result:The linear range was from 0.01 to 0.08 μg, r=0.999 9 (n=5). The average recovery was 100.2% and RSD was 2.7% (n=5). Conclusion: The 5-HMF content increases as the processing time prolongs in a certain time rang.

关键词(KeyWords): 高效液相色谱;地黄;清蒸;5-羟甲基糠醛
HPLC; Radix Rehmanniae; steamed; 5-HMF

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基金项目(Foundation): 国家科技部“十五”攻关重大科研项目(2001BA701A55-22)

作者(Author): 李军,张丽萍,刘伟,张振凌,刘丽娟
LI Jun, ZHANG Li-ping, LIU Wei, ZHANG Zhen-ling, LIU Li-juan(Henan College of Traditional Chinese Medicine, Zhengzhou 450008, China)

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