中国中药杂志

2001, (12)

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高效液相色谱法测定丹参中丹酚酸B
Determination of Salvianolic Acid B in the Radix of Salvia miltiorrhiza Bge. by HPLC

张启伟,张颖,李计萍,马华
ZHANG Qi wei, ZHANG Ying, LI Ji ping, MA Hua (Institute of Chinese Materia Medica,China Academy of TCM,Beijing 100700,China)

摘要(Abstract):

目的 :建立丹参中丹酚酸B高效液相色谱测定方法。方法 :75 %甲醇回流提取 ,色谱柱YWGC18,流动相甲醇 5 %乙酸 (35∶6 5 ) ,检测波长 2 81nm。结果 :丹酚酸B 0 .17~ 1.70 μg线性关系良好 (r =0 .9997) ,回收率98.9% ,RSD1.82 %。结论 :为丹参及其制剂的质量控制提供了分析方法
Objective:To develop an HPLC method for determining salvianolic acid B in radix of Salvia miltiorrhiza .Method:The sample was extracted with 75% methanol. ODS column was used with methanol 5% acetic acid solution ( 35:65 ) as mobile phase. Detection wavelength was 281 nm.Result: Salvianolic acid B was separated well. Good linearity of salvianolic acid B was obtained ( r =0.9997) within the range of0.17~1.7 μg. The average recovery was 98.9%. Repeatability was good, and RSD was 1.82%.Conclusion:This method can be used for quality control of radix of Salvia miltiorrhiza .

关键词(KeyWords): 丹参;丹酚酸B;含量测定;高效液相色谱法
radix of Salvia miltiorrhiza ;salvianolic acid B;quantitative determination;HPLC

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基金项目(Foundation): 国家重点科技项目专题(969030102)

作者(Author): 张启伟,张颖,李计萍,马华
ZHANG Qi wei, ZHANG Ying, LI Ji ping, MA Hua (Institute of Chinese Materia Medica,China Academy of TCM,Beijing 100700,China)

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