郑伟;王芳旭;孙欣光;康利平;赵阳;张洁;马百平;
ZHENG Wei;WANG Fang-xu;SUN Xin-guang;KANG Li-ping;ZHAO Yang;ZHANG Jie;MA Bai-ping;Tianjin University of Traditional Chinese Medicine;Institute of Radiation Medicine,Academy of Military Medical Sciences;National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences;

• 1:天津中医药大学
• 2:军事医学科学院放射与辐射医学研究所
• 3:中国中医科学院中药资源中心

摘要(Abstract)：

中药知母经硫磺熏蒸制备成含硫知母饮片,利用UPLC-Q-TOF-MS^E结合UNIFI数据分析平台、主成分分析法(PCA)及正交偏最小二乘判别分析(OPLS-DA),分析并在线鉴定了硫磺熏蒸前后知母饮片中的16个主要的差异性成分,其中9个主要存在于熏蒸后知母饮片中;确定了熏蒸后知母饮片中的化学标志物,为硫熏后新产生的亚硫酸酯化衍生物。同时利用UPLC-Q-TOF-MSE结合UNIFI数据分析平台、主成分分析法(PCA)及正交偏最小二乘判别分析(OPLS-DA),分析并在线鉴定了硫磺熏蒸前后知母饮片中的16个主要的差异性成分,其中9个主要存在于熏蒸后知母饮片中;确定了熏蒸后知母饮片中的化学标志物,为硫熏后新产生的亚硫酸酯化衍生物。同时利用UPLC-Q-TOF-MS^E寻找主要化学标志物——知母皂苷BⅡ亚硫酸酯(m/z 983)的手段,建立了知母饮片硫磺熏蒸的快速筛查方法,该方法便捷准确,可作为知母质量控制的有效辅助手段。这是首次分析鉴定了甾体皂苷类成分的亚硫酸酯化衍生物,并应用这一方法筛查硫磺熏蒸的知母饮片。
Sulfur-containing Anemarrhenae Rhizoma decoction pieces were prepared by using sulfur-fumigating procedure. The difference components before and after sulfur fumigation in Anemarrhenae Rhizoma were analyzed and on-line identified by UPLC-QTOF-MSE寻找主要化学标志物——知母皂苷BⅡ亚硫酸酯(m/z 983)的手段,建立了知母饮片硫磺熏蒸的快速筛查方法,该方法便捷准确,可作为知母质量控制的有效辅助手段。这是首次分析鉴定了甾体皂苷类成分的亚硫酸酯化衍生物,并应用这一方法筛查硫磺熏蒸的知母饮片。
Sulfur-containing Anemarrhenae Rhizoma decoction pieces were prepared by using sulfur-fumigating procedure. The difference components before and after sulfur fumigation in Anemarrhenae Rhizoma were analyzed and on-line identified by UPLC-QTOF-MS^Ecombined with UNIFI informatics platform,principal component analysis( PCA) and orthogonal partial least squares discriminant analysis( OPLS-DA) respectively. As a result,16 major differences components were identified,and among them,9 components were mainly from sulfur-fumigated samples. The main chemical markers in sulfur-fumigated Anemarrhenae Rhizoma were identified as the sulfite derivatives newly produced after sulfur-fumigating. Meanwhile,UPLC-Q-TOF-MSEcombined with UNIFI informatics platform,principal component analysis( PCA) and orthogonal partial least squares discriminant analysis( OPLS-DA) respectively. As a result,16 major differences components were identified,and among them,9 components were mainly from sulfur-fumigated samples. The main chemical markers in sulfur-fumigated Anemarrhenae Rhizoma were identified as the sulfite derivatives newly produced after sulfur-fumigating. Meanwhile,UPLC-Q-TOF-MS^Ewas used to find the main chemical marker anemarrhena saponin BⅡ sulfite( m/z 983). By using this method,a rapid screening method for sulfur-fumigated Anemarrhenae Rhizoma was established. This was a convenient and accurate detection method for sulfur dioxide residue,and it can be used as an effective assistant method to control the quality of Anemarrhenae Rhizoma. At the same time,it was the first time to identify sulfited derivatives of steroidal saponins,and screen the sulfur-fumigated Anemarrhenae Rhizoma.

关键词(KeyWords)： 知母;硫磺熏蒸;UPLC-Q-TOF-MS~E;化学标志物
Anemarrhenae Rhizoma;sulfur-fumigated;UPLC-Q-TOF-MS~E;chemical markers

Abstract:

Keywords:

基金项目(Foundation):

作者(Authors): 郑伟;王芳旭;孙欣光;康利平;赵阳;张洁;马百平;
ZHENG Wei;WANG Fang-xu;SUN Xin-guang;KANG Li-ping;ZHAO Yang;ZHANG Jie;MA Bai-ping;Tianjin University of Traditional Chinese Medicine;Institute of Radiation Medicine,Academy of Military Medical Sciences;National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences;

DOI: 10.19540/j.cnki.cjcmm.20170719.003

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