中国中药杂志

2013, v.38(07) 1030-1035

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无患子皂苷对自发性高血压大鼠Ang Ⅱ/p38MAPK通路介导的炎症反应与心肌肥厚的影响
Effects of sapindus saponins on inflammatory response mediated by Ang Ⅱ/p38MAPK pathway and cardiac hypertrophy in spontaneously hypertensive rats

陈明;陈志武;龙子江;刘金林;高华武;王雅娟;
CHEN Ming1,2,CHEN Zhi-wu2,LONG Zi-jiang1*,LIU Jin-lin3,GAO Hua-wu1,WANG Ya-juan1(1.Department of Pharmacology,Anhui University of Traditional Chinese Medicine,Hefei 230038,China;2.School of Basic Medicine,Anhui Medical University,Hefei 230031,China;3.Anqing Medical and Pharmaceutical College,Anqing 246052,China)

摘要(Abstract):

目的:通过观察无患子皂苷对Ang Ⅱ/p38MAPK通路介导的自发性高血压大鼠炎症反应的影响,以论证高血压心肌肥厚与炎症因素的相关性。方法:取16周龄SHR32只,随机均分为4组,即SHR模型组、阳性对照组(卡托普利,27mg·kg-1)、无患子皂苷低、高剂量组(27,108mg·kg-1),另取8只健康WKY大鼠作为正常对照组,按剂量连续给药8周,检测指标如下:①左心室质量指数(LVMI)的测定;②ELISA法检测心肌匀浆Ang Ⅱ,hs-CRP的含量;③免疫组化法检测心肌组织AT1R,VEGF的蛋白表达;④Westernblot法检测心肌p-p38MAPK的蛋白表达。结果:无患子皂苷可抑制AngⅡ在心肌局部含量,减少AT1R以及p-p38MAPK的蛋白表达,使炎症因子VEGF,hs-CRP等表达减少,并可降低LVMI,逆转心肌肥厚,上述指标与SHR模型组比较有显著性差异(P<0.05或P<0.01)。结论:无患子皂苷对SHR心肌肥厚有一定的抑制作用,其机制与调控Ang Ⅱ/p38MAPK通路诱导的炎症反应有关。
Objective:To investigate the effects of sapindus saponins on myocardial inflammation mediated by Ang Ⅱ/p38MAPK signal pathway and cardiac hypertrophy in spontaneously hypertensive rats.And also to explore the correlation of cardiac hypertrophy and inflammation.Method:Thirty-two 16-week-old spontaneously hypertensive rats(SHR) were randomly divided into four groups,one with placebo as model group,one with captopril tablets(27 mg·kg-1) as positive control,one with low-dose sapindus saponins(27 mg·kg-1),one with high-dose(108 mg·kg-1).And another eight healthy Wistar-Kyoto strain(WKY) rats were used as the normal group.The animals were treated for eight weeks,and the indicators detected were as follows:①left ventricular mass index(LVMI) ;②the content of AngⅡ and hs-CRP in plasma were determined by ELISA;③the protein expression of AT1R and VEGF were determined by immunohistochemical method;④the protein expression of p-p38MAPK in myocardial cells was determined by Western blot.Result:Sapindus saponins reduced LVMI,and blocked the expression level of AngⅡ,AT1R,p-p38MAPK,VEGF and hs-CRP in myocardial tissue.Vs the SHR model group,there were significant differences(P < 0.05 or P < 0.01).Conclusion:Our findings suggested that sapindus saponins could inhibited cardiac hypertrophy,the possible mechanisms may be related to the inhibition on inflammatory response mediated by AngⅡ/p38MAPK pathway.

关键词(KeyWords): 无患子皂苷;自发性高血压;炎症反应;心肌肥厚
sapindus saponins;spontaneously hypertension;inflammatory response;cardiac hypertrophy

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基金项目(Foundation): 安徽中医学院自然科学基金研究项目(2013Zr006)

作者(Author): 陈明;陈志武;龙子江;刘金林;高华武;王雅娟;
CHEN Ming1,2,CHEN Zhi-wu2,LONG Zi-jiang1*,LIU Jin-lin3,GAO Hua-wu1,WANG Ya-juan1(1.Department of Pharmacology,Anhui University of Traditional Chinese Medicine,Hefei 230038,China;2.School of Basic Medicine,Anhui Medical University,Hefei 230031,China;3.Anqing Medical and Pharmaceutical College,Anqing 246052,China)

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