中国中药杂志

2007, (07) 609-612

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益髓生血颗粒对不同发育阶段小鼠α-血红蛋白稳定蛋白表达的影响
Effect on expression of mice alpha-hemoglobin stabilizing protein in different developmental stages treated with Yisui Shengxue granules

刘咏梅;吴志奎;柴立民;张新华;李敏;陈玉英;吕鑫霞;朱晓云;
LIU Yong-mei1,WU Zhi-kui1,CHAI Li-min2,ZHANG Xin-hua3,LI Min1,CHEN Yu-ying1,LV Xin-xia1,ZHU Xiao-yun1(1.Department of Biology,Guang'anmen Hospital,China Academy of Chinese Medical Sciences,Beijing 100053,China;2.Ophthalmology Hospital,China Academy of Chinese Medical Sciences,Beijing 100053,China;3.Department of Blood,303 Hospital of PLA,Nanning 530021,China)

摘要(Abstract):

目的:探讨益髓生血颗粒对不同发育阶段小鼠α-血红蛋白稳定蛋白(alpha-hemoglob in stabilizing pro-te in,AHSP)表达的影响。方法:分别提取正常成年小鼠骨髓、孕鼠(孕21 d)和胎鼠(孕14 d)胎肝胎脾有核细胞总RNA,采用real-time RT-PCR方法检测中药益髓生血颗粒干预后对不同发育阶段小鼠的α-珠蛋白稳定蛋白基因表达的影响。结果:中药干预后,胎鼠(孕14 d)、孕鼠(孕21 d)的AHSP基因表达无明显改变,而成年鼠骨髓AHSP基因表达明显上调。结论:益髓生血颗粒治疗β-地中海贫血的可能分子机制之一,是通过促进AHSP基因的表达,减少游离α-珠蛋白的沉积,遏止其对红细胞的毒害作用,减少溶血发生而达到治疗效果。
Objective: To discuss the effect of Yisui Shengxue granules on expression of alpha-hemoglobin stabilizing protein(AHSP) mRNA in different developmental stages mice.Method: The total RNAs were extracted from the bone marrow karyocyte of normal adult mice and the karyocyte of fetus liver and fetus spleen in pregnanted mice(pregnanted 21 days) and fetal mice(pregnanted 14 days).The expression level of AHSP mRNA in different developmental stages mice interfered with Yisui Shengxue granules was measured by real-time PCR.Result: The intervention of Yisui Shengxue granules could significantly up-regulated the expression levels of AHSP mRNA in normal adult mice.Conclusion: The result revealed that one of possible molecular mechanism of the effects caused by Yisui Shengxue granules is that it can promote the AHSP gene expression,reduce the free α-globin deposit,then prevent the poison to erythrocyte and decrease the haemolysis.

关键词(KeyWords): 益髓生血颗粒;β-地中海贫血;AHSP;基因表达
Yisui Shengxue granules;β-thalassemia;alpha-hemoglobin stabilizing protein(AHSP);gene expression

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(30171199);; 国家自然科学基金重大研究计划项目(904090033);; 广西自然科学基金项目(桂科自0447104)

作者(Author): 刘咏梅;吴志奎;柴立民;张新华;李敏;陈玉英;吕鑫霞;朱晓云;
LIU Yong-mei1,WU Zhi-kui1,CHAI Li-min2,ZHANG Xin-hua3,LI Min1,CHEN Yu-ying1,LV Xin-xia1,ZHU Xiao-yun1(1.Department of Biology,Guang'anmen Hospital,China Academy of Chinese Medical Sciences,Beijing 100053,China;2.Ophthalmology Hospital,China Academy of Chinese Medical Sciences,Beijing 100053,China;3.Department of Blood,303 Hospital of PLA,Nanning 530021,China)

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