中国中药杂志

2014, v.39(24) 4798-4803

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蒸制和烘制对附子生物碱成分含量的影响研究
Effects of steaming and baking on content of alkaloids in Aconite Lateralis Radix(Fuzi)

杨昌林;黄志芳;张意涵;刘玉红;刘云华;陈燕;易进海;
YANG Chang-lin;HUANG Zhi-fang;ZHANG Yi-han;LIU Yu-hong;LIU Yun-huan;CHEN Yan;YI Jin-hai;Sichuan Academy of Chinese Medicine Sciences;Chengdu University of Traditional Chinese Medicine;

摘要(Abstract):

研究蒸制和烘制对附子生物碱含量的影响,采用UPLC-MS/MS方法,多反应监测模式(MRM)同时测定附子中13种生物碱成分的含量。结果表明蒸制过程中附子双酯型生物碱含量迅速降低,单酯型生物碱含量先升后降,于40 min时达到最高峰,原碱中乌头原碱、新乌头原碱、次乌头原碱快速增加,附子灵、宋果灵、多根乌头碱和去甲猪毛菜碱含量相对稳定或略有降低。烘制过程中生物碱成分的动态变化趋势与蒸制过程有明显差异,双酯型生物碱降解速度比蒸制稍慢,单酯型生物碱、原碱和总生物碱被不同程度破坏,含量显著低于同时间点蒸制附片。该研究揭示了附子蒸制或烘制过程中生物碱成分的动态变化规律,2种炮制方法均能有效去除毒性成分并保留有效成分,工艺简便易行,方法可控,具有推广和应用价值。
To study the effect of steaming and baking process on contents of alkaloids in Aconite Lateralis Radix(Fuzi),13 alkaloids were analyzed by UPLC-MS / MS equipped with ESI+ion source in MRM mode.In steaming process,the contents of diesterditerpenoid alkaloids decreased rapidly,the contents of monoester-diterpenoid alkaloids firstly increased,reached the peak at 40 min,and then deceased gradually.The contents of aconine alkaloids(mesaconine,aconine and hypaconine) increased all the time during processing,while the contents of fuziline,songorine,karacoline,salsolionl were stable or slightly decreased.In baking process,dynamic variations of alkaloids were different from that in the steaming process.Diester-diterpenoid alkaloids were degraded slightly slower than in steaming process.Monoester-diterpenoid alkaloids、aconine alkaloids and the total alkaloids had been destroyed at different degrees,their contents were significantly lower than the ones in steaming Fuzi at the same processing time.This experiment revealed the dynamic variations of alkaloids in the course of steaming and baking.Two processing methods which can both effectively remove the toxic ingredients and retain the active ingredients are simple and controllable,and are valuable for popularization and application.

关键词(KeyWords): 附子;生物碱;UPLC-MS/MS;炮制;变化规律
Aconite Lateralis Radix;alkaloid;UPLC-MS/MS;processing;variation

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基金项目(Foundation): 国家重点基础研究发展计划(973)项目(2009CB522804);; 国家科技支撑计划项目(2011BAI13B05)

作者(Author): 杨昌林;黄志芳;张意涵;刘玉红;刘云华;陈燕;易进海;
YANG Chang-lin;HUANG Zhi-fang;ZHANG Yi-han;LIU Yu-hong;LIU Yun-huan;CHEN Yan;YI Jin-hai;Sichuan Academy of Chinese Medicine Sciences;Chengdu University of Traditional Chinese Medicine;

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