中国中药杂志

2008, (22) 2658-2662

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芍药甘草复方效应组分谱效关系研究
Study on fingerprints correlated with pharmacodynamic of Paeonia lacliflora and Glycyrrhiza uralensis effective compounds

沈岚;张梁;冯怡;徐德生;林晓;
SHEN Lan,ZHANG Liang,FENG Yi,XU De-sheng,LIN Xiao(1.School of Pharmacy,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;2.Technology Center,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;3.Shuguang Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai 200021,China)

摘要(Abstract):

目的:研究芍药甘草复方大鼠给药后的解痉活性成分。方法:在建立芍药甘草体内成分指纹图谱分析方法的基础上,通过对芍药甘草各效应组分8组不同配比给药后大鼠血清的胃底肌条解痉实验,获得各组抑制率,将药效学信息与各组血清HPLC指纹图谱化学信息进行相关性研究。并采用HPLC-ESI-MS/MS分析芍药甘草效应组分给药后血清样品。结果:谱效相关性研究发现,芍药甘草效应组分给药后体内4个色谱峰与药效相关性较大,与药效呈正相关,且相关系数大于0.4,分别为0.774,0.678,0.566,0.472,其保留时间分别为8.03,51.7,54.59,72.43 min;经质谱分析,主要为效应组分代谢成分。结论:通过谱效研究直接获得体内活性物质,建立了体内活性物质谱效研究的一种新方法,是复方中药的药效物质基础确定的有效手段。
Objective: To study the spasmolysisy activity constituents of the Radix Paeoniae alba and Radix et Rhizoma compound.Method: The three effective compounds of Radix Paeoniae and Radix et Rhizoma were arrayed to eight groups by orthogonal design rat,serum samples were collected after administered in these groups,and these serum samples were used to carry out the experiments of serum pharmacology of spasmolysis.The inhibition ratios were assigned as pharmacodynamic variable X,the areas of characteristic peaks on HPLC fingerprints were assigned as variable Y,and the correlation of X and Y was studied in order to research the activity spasmolysisy compounds of effective constituents.Serum samples were analyzed by HPLC-ESI-MS/MS.Result: The correlation of fingerprints and pharmacology showed that four compounds were the main spasmolysisy activity compounds.The correlation parameter was greater than>0.4,whose value were 0.774,0.678,0.566 and 0.472 respectively,and their retention times were 8.03,51.7,54.59 and 72.43 min respectively.They were metabolites of effective compounds.Conclusion: This study makes a valid approach for understanding the material foundation of TCM by establishing the correlation of fingerprints and pharmacology of activity compounds in vivo.

关键词(KeyWords): 芍药甘草;谱效;物质基础
Paeonia lacliflora and Glycyrrhiza uralensis;fingerprints-pharmacology;material foundation

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金青年科学基金(30801548);; 上海市青年科技启明星计划A类基金(08QA14060);; 上海市教委重点学科基金(J50302);; 高等学校博士学科点专项科研基金(20060268009)

作者(Authors): 沈岚;张梁;冯怡;徐德生;林晓;
SHEN Lan,ZHANG Liang,FENG Yi,XU De-sheng,LIN Xiao(1.School of Pharmacy,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;2.Technology Center,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;3.Shuguang Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai 200021,China)

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