中国中药杂志

2010, v.35(19) 2541-2545

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

HPLC-UV-ELSD同时测定大鼠尿样中人参皂苷和淫羊藿黄酮苷类成分
Simultaneous determination of ginsenosides and epimedium flavonoids in rat urine by HPLC-UV-ELSD

刘敏彦;张兰桐;赵韶华;王宏涛;王巧;
LIU Minyan1,2, ZHANG Lantong1, ZHAO Shaohua2, WANG Hongtao2, WANG Qiao1(1.Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University, Shijiazhuang 050017, China;2. Shijiazhuang Yiling Pharmaceutical Co., Ltd., Shijiazhuang 050035, China)

摘要(Abstract):

目的:建立HPLC紫外检测器串联蒸发光散射检测器的方法同时测定大鼠尾静脉注射肌萎灵冻干粉后尿样中人参皂苷和淫羊藿黄酮苷类成分。方法:采用C18色谱柱,流动相乙腈-水,梯度洗脱,紫外检测器:检测波长220nm;蒸发光散射检测器:漂移管温度80℃,气体压力172.4kPa。结果:所检测的12种成分在检测的范围内线性关系良好,R2均大于0.998,提取回收率在88.67%~101.2%,日内和日间精密度的RSD小于9.1%,RE在-11.58%~10.89%。结论:该方法专属性好,精密度好,可用于大鼠尾静脉注射肌萎灵冻干粉后尿样中皂苷和黄酮苷类成分的测定,该结果可为肌萎灵冻干粉的体内药物代谢和临床用药提供参考。
Objective:To develop and validate a HPLC-UV-ELSD method for the simultaneous determination of ginsenosides and epimedium flavonoids in rat urine after intravenous administration of Jiweiling freeze-dried powder. Method: Chromatographic separation was performed on a C18 HPLC column, with gradient elution of acetonitrile and water as mobile phase. An UV detector was used at detection wavelength of 220 nm. An evaporative light scattering detector(ELSD)was used at drift tube temperature of 80 ℃ and gas pressure of 172.4 kPa. Result: The calibration curves were linear over the investigated concentration ranges with all correlation coefficients higher than 0.998. The a intra-and inter-day RSD were less than 9.1% and the relative errors were verage extraction recoveries for all compounds were between 88.67% and 101.2%. Thewithin the range of -11.58% to 10.89%. Conclusion: The proposed method showed appropriate accuracy and selectivity and was successfully applied to the rat urine samples analysis of saponins and flavonoids after intravenous administration of Jiweiling freeze-dried powder, which may provide some references to the apprehension of the action mechanism and clinical application.

关键词(KeyWords): 肌萎灵冻干粉;人参皂苷;淫羊藿黄酮苷;HPLC-UV-ELSD;尿样测定
Jiweiling freeze-dried powder; ginsenosides; epimedium flavonoids; HPLC-UV-ELSD; determination of rat urine

Abstract:

Keywords:

基金项目(Foundation): 国家“重大新药创制”科技重大专项(2009ZX09313-003,2009ZX09102-140)

作者(Author): 刘敏彦;张兰桐;赵韶华;王宏涛;王巧;
LIU Minyan1,2, ZHANG Lantong1, ZHAO Shaohua2, WANG Hongtao2, WANG Qiao1(1.Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University, Shijiazhuang 050017, China;2. Shijiazhuang Yiling Pharmaceutical Co., Ltd., Shijiazhuang 050035, China)

Email:

DOI:

扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享