中国中药杂志

2018, v.43(24) 4884-4891

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酸枣仁中化学成分的UHPLC-LTQ-Orbitrap-MS快速分析
Rapid analysis of chemical components in Zizyphi Spinosae Semen by UHPLC-LTQ-Orbitrap-MS

毛怡宁;武文奇;康莹;王方方;刘勇;
MAO Yi-ning;WU Wen-qi;KANG Ying;WANG Fang-fang;LIU Yong;Beijing University of Chinese Medicine;

摘要(Abstract):

基于UHPLC-LTQ-Orbitrap-MS技术对酸枣仁中化学成分进行定性分析。采用Waters Acquity UPLC BEH-C18色谱柱(2. 1 mm×50 mm,1. 7μm),以乙腈(A)-0. 1%甲酸水溶液(B)为流动相梯度洗脱,体积流量0. 3 m L·min-1;在电喷雾负离子模式下采集数据。将母离子列表(parent ion list,PIL)-MS2和高能碰撞(high energy collision dissociation,HCD)技术,用于酸枣仁中三萜皂苷成分的鉴别。结合质谱数据和文献数据,共鉴定、推断出24个化合物,4个未知化合物;其中包括21个黄酮类化合物,5个皂苷类化合物,2个三萜类化合物。采用UHPLC-LTQ-Orbitrap-MS技术,为酸枣仁中化学成分的定性分析及质量控制提供了一种高效、快速的分析方法,为今后进一步阐明其药效物质基础及资源开发利用提供了数据基础。
This study aimed to analyze the chemical components of Zizyphi Spinosae Semen by using ultra high performance liquid chromatography-linear ion trap/orbitrap high resolution mass spectrometry( UHPLC-LTQ-Orbitrap-MS). The chromatographic separation of the components was achieved on a Waters Acquity UPLC BEH-C18 column( 2. 1 mm × 50 mm,1. 7 μm),with acetonitrile-water( 0. 1% formic acid) as mobile phase at a flow rate of 0. 3 m L·min-1. Electrospray ionization-( ESI) source was applied and operated in negative ion mode. The parent ion list( PIL)-MS2 and high energy collision dissociation( HCD) techniques were used to identify triterpene saponins in Zizyphi Spinosae Semen. Through analysis of the mass spectrometry data and literature data,24 compounds and 4 unknown compounds were identified and inferred,including 21 flavonoids,5 saponins and 2 triterpenoids. The use of UHPLC-LTQ-Orbitrap-MS technology provide an efficient and rapid analytical method for the qualitative analysis and quality control of the chemical components in Zizyphi Spinosae Semen. It can also provide reference data for the research on the material basis of pharmacodynamics and the resources development and utilization.

关键词(KeyWords): 酸枣仁;UHPLC-LTQ-Orbitrap-MS;化学成分
Zizyphi Spinosae Semen;UHPLC-LTQ-Orbitrap-MS;chemical components

Abstract:

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基金项目(Foundation):

作者(Authors): 毛怡宁;武文奇;康莹;王方方;刘勇;
MAO Yi-ning;WU Wen-qi;KANG Ying;WANG Fang-fang;LIU Yong;Beijing University of Chinese Medicine;

DOI: 10.19540/j.cnki.cjcmm.20180915.001

参考文献(References):

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