中国中药杂志

2008, (13) 1523-1527

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石蒜属叶绿体trnL-F序列的变异与系统聚类分析
Genetic variations in trnL-F sequence and phylogenetic clustering of Lycoris species

袁菊红;孙视;彭峰;冯煦;郑玉红;夏冰;
YUAN Ju-hong~(1,2) SUN Shi~1 PENG Feng~1 FENG Xu~1 ZHENG Yu-hong~1 XIA Bing~1 (1.Institute of Botany,Jiangsu Province & Chinese Academy of Sciences Nanjing Botanical Garden Mem.Sun Yat-sen,Nanjing 210014,China;2.Resource and Environment Management Institute,Jiangxi University of Finance and Economics,Nanchang 330032,China)

摘要(Abstract):

目的:分析比较石蒜属和近缘属植物中国水仙trnL基因的部分序列和trnL-trnF间隔区的完整序列,为石蒜属物种鉴别和种间亲缘关系分析提供分子佐证。方法:采用PCR产物直接双向测序法,用Clustal X 1.81,MEGA 2.0软件进行序列分析,采用最大简约法(maximum parsimony,MP)构建系统发育树。结果:所测物种的序列全长在905~1036 bp,经排序后,两端切平,得到886 bp的整齐序列,所测物种的核苷酸变异位点14个,信息位点10个,主要在trnL内含子区,这些位点可用于区分石蒜属物种;4个碱基"ATAT"缺失/插入是区别石蒜属与其近缘植物水仙的显著特征。重建的系统发育树表明:供试的石蒜属物种聚成3类,除稻草石蒜、忽地笑、香石蒜的系统位置有所不同之外,与经典分类结果基本相符。水仙属2个种形成1个单系类群,表明属间trnL-F序列差异明显。结论:分子系统树支持安徽石蒜是长筒石蒜的变种或杂交种观点,换锦花可能是玫瑰石蒜、红蓝石蒜的杂交母本。石蒜属种间trnL-F序列存在一定变异,是物种鉴别的有效分子标记。
Objective:To identify some closely related Lycoris species and evaluate interspecific relationships among them. Method:The cpDNA trnL-F sequence of 20 taxa representing 15 species of Lycoris and Narcissus tazaetta var.chinensis as one out- group were determined by using direct sequencing of PCR product,and they were analyzed by means of the software of CLUSTRAL and MEGA.Result:The length of trnL-F of all taxa was 905~1036 bp.When the gaps were always treated as missing,there were 14 var- iable sites and 10 parsim-info sites,which could be used to identify some species of Lycoris.Four nucleotides inserteions/deletions were significant different among Lycoris and two species of Narcissus.Phylogeny tree was constructed with the maximum parsimony methods by bootstrap test.Three infrageneric clades of all Lycoris species were resolved.The classification was basically consistent with that of morphology except for L.longituba,L aurea,and L straminea.Conclusion:The tree suggested that L.anhuiensis can not be taken as an independent species,while it may be of a variety or a hybrid of L longituba.Regarding the hybrid origin species,the ma- teral parent of L.rosea and L.haywardii was revealed to be L sprengeri.There were some variations in the trnL-F sequence,which were good molecular markers for identification species of Lycoris.

关键词(KeyWords): 石蒜属;tmL-F序列;序列变异;分子鉴别;系统树
Lycoris;trnL-F sequences;nucleotide variation;molecular identification;phylogenetic tree

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(30370292);; 江苏省科技基础设施建设计划项目(BM2006104)

作者(Author): 袁菊红;孙视;彭峰;冯煦;郑玉红;夏冰;
YUAN Ju-hong~(1,2) SUN Shi~1 PENG Feng~1 FENG Xu~1 ZHENG Yu-hong~1 XIA Bing~1 (1.Institute of Botany,Jiangsu Province & Chinese Academy of Sciences Nanjing Botanical Garden Mem.Sun Yat-sen,Nanjing 210014,China;2.Resource and Environment Management Institute,Jiangxi University of Finance and Economics,Nanchang 330032,China)

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