中国中药杂志

2008, (15) 1865-1868

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雾化吸入银杏叶总黄酮对哮喘小鼠Th1/Th2失衡的影响
Effect of nebulized TFG on Th1/Th2 imbalance in mouse model with asthma

陈莉莉;翁晓静;李心;张洪泉;
CHEN Li-li,WENG Xiao-jing,LI Xin,ZHANG Hong-quan(Medical and Pharmaceutical Institute of Yangzhou University,Yangzhou 225001,China)

摘要(Abstract):

目的:研究银杏叶总黄酮(total ginkgo flavone glycosides,TFG)对哮喘小鼠辅助性T细胞1/辅助性T细胞2(Th1/Th2)失衡的影响。方法:48只BALB/c小鼠随机分为正常组、哮喘组、TGF雾化吸入组以及地塞米松组,以卵蛋白(OVA)、氢氧化铝免疫系统致敏和局部激发的方法建立哮喘模型。自实验第21天起给予TFG治疗组连续雾化吸入50 g.L-1TFG溶液30 min,地塞米松组腹腔注射地塞米松1 mg.kg-1,连续10 d。第32天酶联免疫吸附实验(enzyme-linked immunosorbent assay,ELISA)法测定肺细胞灌洗液(bronchalveolar lavage fluid,BALF)中细胞因子γ-干扰素(interferon-γ,IFN-γ)、白介素-4(interleukin-4,IL-4)和血清中总免疫球蛋白E(immunoglobulinE,IgE)含量,观察各组肺组织的病理变化,免疫组织化学染色技术检测肺组织中GATA连接蛋白-3(GATA bindingprotein-3,GATA-3)的表达情况。结果:与模型组比较,TFG组BALF中IL-4含量(49.30±7.95)ng.L-1降低(P<0.01)、IFN-γ水平升高(49.08±5.46)ng.L-1(P<0.01)、血浆总IgE水平降低(9.47±1.52)μg.L-1(P<0.01);模型组支气管平滑肌肥厚,黏膜充血、水肿,管腔内可见黏液栓,并有大量炎性细胞浸润;而TFG组和地塞米松组小鼠的上述炎症性改变明显减轻;模型组GATA-3表达显著,TFG治疗组则明显减轻。结论:雾化吸入TFG对哮喘小鼠有明显的治疗作用,其机制可能与阻断GATA-3的表达、恢复Th1/Th2平衡有关。
Objective:To investigate the effect of nebulized total ginkgo flavone glycosides(TFG) on Th1/Th2 imbalance in mice with athma.Method:Forty-eight BALB/C mice were randomly divided into four groups:group A(control group,n=12);group B(asthmatic model group,n=12);group C(TFG nebulized treated group,n=12);group D(dexamethasone intraperitoneal treated group,n=12).The asthmatic model was established by sensitivity and local activation with Ovalbumin(OVA) and aluminum hydroxide Al(OH)3.TFG(50 g·L-1,per aerosol per six mice,30 minutes) was nebulized 20 days after modeling,while dexamethasone(1 g·L-1) was intraperitoneal once daily for 10 days.Perfusate of bronchoalveolar lavage fluid(BALF) was collected on day 32.The level of IL-4,IFN-γ in BALF,and the level of total IgE in serum was determined.The airway inflammation pathology change and the expression of GATA-3 protein in lungs was detected by immunohistochemical staining.Result:Compared with model group,the decreased content of IL-4(49.30±7.95) ng·L-1 and increased level of IFN-γ(49.08±5.46) ng·L-1 were found in BALF,and the level of total IgE(9.47±1.52) μg·L-1 in serum also decreased in TFG treated group.In model group,smooth muscle hypertrophing,mucous hyperemia,mucous layer thickening,and inflammatory cell in filtration were observed.Phlegmasia was appeared in the bronchi,which was filled with lots of mucus.In contrast,the inflammatory reaction in TFG and Dexamethasone treated group was less obvious.Expression of GATA-3 was markly increased in model group with decreased expression in TFG treated group.Conclution:Nebulized TFG showed a therapeutic effect for asthmatic mice,the mechanism may be explained by blockingnnnn GATA-3 expression and regulating the disorder Th1/Th2 imbalance.

关键词(KeyWords): 银杏叶总黄酮;哮喘;Th1/Th2;雾化吸入
total ginkgo flavone glycosides;asthma;Th1/Th2;nebulization

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作者(Author): 陈莉莉;翁晓静;李心;张洪泉;
CHEN Li-li,WENG Xiao-jing,LI Xin,ZHANG Hong-quan(Medical and Pharmaceutical Institute of Yangzhou University,Yangzhou 225001,China)

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