中国中药杂志

2016, v.41(10) 1797-1802

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野生关黄柏中生物碱及绿原酸含量区域性特征分析
Regional characteristic analysis of alkaloids and chlorogenic acid in wild Phellodendri Amurensis Cortex

张阳;张志鹏;张昭;毕成军;刘海涛;张琪;黄少雄;张本刚;陈瑶;索风梅;
ZHANG Yang;ZHANG Zhi-peng;ZHANG Zhao;BI Cheng-jun;LIU Hai-tao;ZHANG Qi;HUANG Shao-xiong;ZHANG Ben-gang;CHEN Yao;SUO Feng-mei;Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine,Ministry of Education,Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences,Peking Union Medical College;Nationalized Fushun Country Three Stone Forest Farm;

摘要(Abstract):

该研究在关黄柏基源植物黄檗的整个分布区内,以每一个纬度为间隔设置采样点,在控制采样植株茎粗、采样部位及采样时间的前提下,于31个样点共采集674份野生关黄柏药材样本。对样本中盐酸巴马汀、盐酸小檗碱、盐酸黄柏碱、盐酸药根碱、木兰花碱和绿原酸等6种活性成分的含量进行测定发现,药材中6种活性成分的含量因采样点的不同存在显著差异,辽宁地区所产药材6种活性成分含量最高,其次为北京和吉林地区,黑龙江地区药材的含量最低,研究结果对探究关黄柏药材活性成分形成的环境因素及人工种植区划具有重要的指导意义。
In the present research,674 wild medicinal material samples of Phellodendri amurensis Cortex were collected from 31 sampling sites in the whole distribution of its original plant Phellodendron amurense. The samples were collected under the premise that the stem diameter of sampling plant,sampling position and time were controlled. And the sampling sites were set at the interval of a latitude. The content of 6 kinds of active ingredients,palmatine chloride,berberine hydrochloride,phellodendrine chloride,jatrorrhizine hydrochloride,magnoflorine,chlorogenic acid,etc in the medicinal material samples were determined,and the results showed that the content of most active ingredients in the medicinal materials showed significant differences due to the difference of sampling sites.Among them,the medicinal materials from Liaoning region had the highest content of active ingredients,followed by Beijing and Jilin regions,and that from Heilongjiang region had the lowest content. The study has important directive significance to the exploration of environmental factors for the formation of active constituent and artificial planting regionalization of high quality Phellodendri amurensis Cortex.

关键词(KeyWords): 关黄柏;黄檗;活性成分;区域性特征;差异性分析;聚类分析
Phellodendri Amurensis Cortex;Phellodendron amurense;active constituent;regional characteristic;difference analysis;clustering analysis

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81473305)

作者(Authors): 张阳;张志鹏;张昭;毕成军;刘海涛;张琪;黄少雄;张本刚;陈瑶;索风梅;
ZHANG Yang;ZHANG Zhi-peng;ZHANG Zhao;BI Cheng-jun;LIU Hai-tao;ZHANG Qi;HUANG Shao-xiong;ZHANG Ben-gang;CHEN Yao;SUO Feng-mei;Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine,Ministry of Education,Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences,Peking Union Medical College;Nationalized Fushun Country Three Stone Forest Farm;

参考文献(References):

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