中国中药杂志

2004, (08)

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若干中草药中25种元素在不同浸取液中的分布
Preliminary attempt at the speciation of 25-elements in the chinese medicinal herbs

王京宇,欧阳荔,刘雅琼,解清,黄卓,屠鹏飞,郭绪林,刘虎生
WANG Jing-yu~1, OUYANG Li~1, LIU Ya-qiong~1, XIE Qing~1, HUANG Zhuo~1, TU Peng-fei~2, GUO Xu-lin~2, LIU Hu-sheng~1,(1.School of Public Health, Peking University, Beijing 100083, China; 2.School of Pharmaceutical Sciences, Peking University, Beijing 100083, China)

摘要(Abstract):

目的 :通过测定不同浸取液中 2 5种元素含量 ,探讨这些元素在中草药中的存在形态。方法 :对同一产地的 5种不同中草药密蒙花Buddlejaofficinalis、白鲜皮Dictamnusdasycarpus、肉豆蔻Myristicafragrans、合欢Albiziajulibrissin、旋覆花Inulajaponica进行水提、二氯甲烷浸取、残渣消化 ,分别得到水溶相、脂溶相、不溶相 ;参照药典方法对我国 9个不同产地的商陆Phytolaccaacinosa进行了 0 %乙醇、5 0 %乙醇、75 %乙醇和 95 %乙醇的浸取处理 ;使用ICP MS(电感耦合等离子体质谱仪 )测定上述三相及各个乙醇浸取液中铍、镉、铈、铬、铜、镝、铕、铒、钆、锗、钬、镧、镥、钼、钕、铅、镨、钐、锶、铽、铊、铥、钇、镱和锌共 2 5种元素的含量。结果 :实验表明 ,用不同溶剂浸取中草药时微量元素的浸取率有较大差异 ,并以有机态与无机态形式共存。在优化实验条件下 ,ICP MS能够同时测定中草药中的2 5种元素 ,其方法检出限为 0 .0 0 3~ 0 .71μg·L-1。中草药中元素的加标回收率为 88%~ 119% ,相对标准偏差为1 .7%~ 13.3%。结论 :上述结果对于在中成药生产过程中尽可能地浸取人体必须元素、除去有毒元素、促进中药出口具有一定的指导意义
Objective: To make an attempt at the multi-element speciation in the chinese medicinal herbs by determining the concentrations of 25 elements in different extraction solutions. Method: Firstly, five chinese medicinal herbs (Buddleja officinalis, Dictamnus dasycarpus, Myristica fragrans, Albizia julibrissin and Inula japonica) from the same region of China were treated to obtain water-soluble phase, lipid-soluble phase and non-soluble phase by water extraction, organic solvent extraction and acid digestion, respectively. Secondly, Phytolacca acinosa, a chinese medicinal herb collected from 9 regions of China, was extracted by 0%EtOH, 50%EtOH, 75%EtOH, 95%EtOH, respectively, referring the Chinese Pharmacopoeia. Finally, the concentrations of 25 elements, such as Be, Cr, Cu, Zn, Ge, Sr, Y, Mo, Cd, Tl, Pb and REEs, in the above three phases were determined by ICP-MS. Result: Under the optimal conditions, all the 25 elements could be determined with detection limits ranged from 0.003 to 0.71 ng·g~(-1). The average recoveries of the elements in P. acinosa were 88%~119%, with the relative standard deviations 1.7%~13.3%. It was observed that the determined 25 elements distributed in all the water-soluble, lipid-soluble and non-soluble phases, indicating that the inorganic species, organicspecies, as well as the protein bound species were co-existed in the herbs. Big differences of the element extraction rates could be found by using different ethanol solutions. Conclusion: With the aid of the obtained results, we may increase the extraction of necessary elements while decrease that of the toxic elements from the herbs by choosing a suitable solvent during the drug production.

关键词(KeyWords): 中草药;浸取;微量元素;稀土元素;ICPMS;形态
Chinese medicinal herbs; extraction; trace elements; rare earth elements; ICP-MS; speciation

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作者(Author): 王京宇,欧阳荔,刘雅琼,解清,黄卓,屠鹏飞,郭绪林,刘虎生
WANG Jing-yu~1, OUYANG Li~1, LIU Ya-qiong~1, XIE Qing~1, HUANG Zhuo~1, TU Peng-fei~2, GUO Xu-lin~2, LIU Hu-sheng~1,(1.School of Public Health, Peking University, Beijing 100083, China; 2.School of Pharmaceutical Sciences, Peking University, Beijing 100083, China)

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