中国中药杂志

2016, v.41(04) 737-742

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通脉颗粒在急性心肌缺血模型比格犬体内药动学研究
Pharmacokinetics of Tongmai granule in acute myocardialischemic model beagles

张颖;刘春旭;李磊;杨秀伟;刘东春;林成仁;刘建勋;
ZHANG Ying;LIU Chun-xu;LI Lei;YANG Xiu-wei;LIU Dong-chun;LIN Cheng-ren;LIU Jian-xun;Institute of Basic Medical Sciences,Xiyuan Hospital,China Academy of Chinese Medical Sciences,Beijing Key Laboratory of Pharmacology of Chinese Material Medica;Department of Pharmacognosy,School of Traditional Chinese medicine,Shenyang Pharmaceutical University;State Key Laboratory of Natural and Biomimetic Drugs,School of Pharmaceutical Sciences,Peking University;

摘要(Abstract):

通脉颗粒(TM)是由丹参、川芎、葛根组方而成,临床上用于对缺血性心脑血管疾病的治疗。为研究TM的药效物质基础,对急性心肌缺血模型比格犬给予TM干预,分析了血清中吸收成分及其药动学特征。以盐酸酸化-乙酸乙酯萃取法提取血清样品,在LS-MS/MS的ESI-检测模式下,采用Merck RP-18e整体反相硅胶色谱柱分离,以乙腈-甲醇1∶1(0.07%FA)和水(0.01%FA)为流动相,在优化的梯度洗脱程序下实现了在8.5 min内对犬血清中8种葛根黄酮成分、5种丹参酚酸成分和2种葛根成分的同时定量分析。分析方法的验证结果表明该方法特异、准确、精密,符合生物分析要求。对急性心梗模型比格犬十二指肠注射TM后6 h内不同时间点血清样品的分析结果显示,给药后5 min药物成分即吸收入血,15种成分均能在血清中检出,分别于给药后0.183.83 h达峰。各成分的血药浓度差异很大,其中葛根素、丹参素体内暴露量远高于其他成分,其次为3'-甲氧基葛根素、芹糖基葛根素和3'-羟基葛根素。研究结果表明葛根异黄酮类和丹参素是TM在心肌缺血模型犬体内的主要活性成分。
Tongmai granule( TM) is composed of Puerariae Lobatae Radix( Gegen),Salviae Miltiorrhizae Radix et Rhizoma( Danshen) and Chuanxiong Rhizoma( Chuanxiong). It has been used to treat ischemic cardio-cerebrovascular diseases for decades. For the purpose of elucidating its pharmacodynamic material foundation,the absorption and pharmacokinetic property of TM were investigated in acute myocardial ischemic model beagles. All serum samples were extracted before analysis with ethyl acetate after being acidified by hydrochloric acid. Under negative ESI detection mode,the chromatographic separation was carried out with monolithic C18 column for gradient elution. A simultaneous quantitative analysis was made on 15 polyphenols,including 8 from Gegen,5 from Danshen and 2from Chuanxiong,in 8. 5 min. The validation result demonstrated the specificity,accuracy and precision of the method in line with the bioanalysis requirements. After TM solution was administrated to acute myocardial ischemic model beagles through duodenum injection,serum samples were collected after 6 h. The quantitative detection proved the prompt absorption of TM,all of the components were detectable in the blood samples 5 min later,and reached peak respectively at 0. 18-3. 83 h after administration. The components presented large variabilities. The most components were exposed in serum with puerarin and salvianic acid A,followed by 3'-methoxypuerarin,mirificin,and 3'-hydroxypuerarin. The study proves puerarin and salvianic acid A are dominating active components of TM in acute myocardial ischemic model beagles.

关键词(KeyWords): 通脉颗粒;急性心肌缺血模型犬;药动学;LC-MS/MS;多酚;血药浓度
Tongmai granule;acute myocardial ischemic model beagles;pharmacokinetics;LC-MS/MS;polyphenols;serum concentration

Abstract:

Keywords:

基金项目(Foundation): 国家“重大新药创制”科技重大专项(2009ZX09502-006);; 国家重点基础研究发展计划(973)项目(2015CB554405)

作者(Authors): 张颖;刘春旭;李磊;杨秀伟;刘东春;林成仁;刘建勋;
ZHANG Ying;LIU Chun-xu;LI Lei;YANG Xiu-wei;LIU Dong-chun;LIN Cheng-ren;LIU Jian-xun;Institute of Basic Medical Sciences,Xiyuan Hospital,China Academy of Chinese Medical Sciences,Beijing Key Laboratory of Pharmacology of Chinese Material Medica;Department of Pharmacognosy,School of Traditional Chinese medicine,Shenyang Pharmaceutical University;State Key Laboratory of Natural and Biomimetic Drugs,School of Pharmaceutical Sciences,Peking University;

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