中国中药杂志

2017, v.42(03) 523-530

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茵陈提取物中化学成分的UHPLC-LTQ-Orbitrap快速鉴定
Rapid characterization of chemical constituents in capillary wormwood extract based on UHPLC-LTQ-Orbitrap

欧阳文竹;尚展鹏;王文建;王守旭;沈家序;钱浩;马志国;张加余;
OUYANG Wen-zhu;SHANG Zhan-peng;WANG Wen-jian;WANG Shou-xu;SHEN Jia-xu;QIAN Hao;MA Zhi-guo;ZHANG Jia-yu;Department of Biological and Chemical Engineering,Bozhou University;Beijing Research Institution of Chinese Medicine,Beijing University of Chinese Medicine;Huatuo Medicines Company Limited;Bozhou Huqiao Medicines Company Limited;College of Pharmacy,Jinan University;

摘要(Abstract):

应用UHPLC-HR-MSn技术分析检测茵陈提取物中的化学成分。采用ACQUITY UPLC HSS T3(2.1 mm×100 mm,1.7μm)色谱柱和0.1%甲酸水溶液(A)-乙腈(B)流动相系统进行梯度洗脱;应用LTQ-Orbitrap质谱负离子模式检测茵陈提取物中的化学成分。根据精确分子量数据和多级质谱碎片离子,结合对照品比对和文献报道,共从茵陈提取物中分析鉴定了50个化学成分,包括21个黄酮、22个有机酸、6个香豆素和1个其他类成分。应用UHPLC-LTQ-Orbitrap高分辨液质联用技术较为全面地阐明了茵陈提取物中的化学成分,为进一步研究茵陈提取物的全面质量控制方法、体内代谢过程以及药效物质基础提供了科学依据。
A rapid and sensitive UHPLC-HR-MSnmethod was developed for the identification of chemical constituents in capillary wormwood extract. ACQUITY UHPLC HSS T3 chromatography column( 2. 1 mm × 100 mm,1. 7 μm) was used with 0. 1% formic acid-acetonitrile solution as the mobile phase in gradient elution. The extract was detected by ESI-LTQ-Orbitrap equipped with an ESI ion source in a negative mode. Based on the accurate mass measurements,retention time,mass fragmentation patterns and literature reports,a total of 50 compounds including 21 flavonoids,22 phenolic acids,6 coumarins and 1 other compound were tentatively screened and characterized. These results are helpful for the comprehensive quality control,better comprehension of the metabolism and further study of pharmacodynamic substance from capillary wormwood extract.

关键词(KeyWords): 茵陈提取物;液质联用技术;化学成分鉴定
capillary wormwood extract;UHPLC-LTQ-Orbitrap MS;chemical constituent identification

Abstract:

Keywords:

基金项目(Foundation): 亳州市创新创业领军人才行动计划“中药复方产品开发科研团队”项目;; 亳州学院“中药复方产品开发创新团队”项目;亳州学院校企合作技术创新平台“中药质量评价工程技术研究中心”项目(2016xqzx06)

作者(Authors): 欧阳文竹;尚展鹏;王文建;王守旭;沈家序;钱浩;马志国;张加余;
OUYANG Wen-zhu;SHANG Zhan-peng;WANG Wen-jian;WANG Shou-xu;SHEN Jia-xu;QIAN Hao;MA Zhi-guo;ZHANG Jia-yu;Department of Biological and Chemical Engineering,Bozhou University;Beijing Research Institution of Chinese Medicine,Beijing University of Chinese Medicine;Huatuo Medicines Company Limited;Bozhou Huqiao Medicines Company Limited;College of Pharmacy,Jinan University;

DOI: 10.19540/j.cnki.cjcmm.2017.0010

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