丁艳霞;王腾宇;张耀文;黄玉梅;马琳;李冬冬;窦德强;李钦;
DING Yan-xia;WANG Teng-yu;ZHANG Yao-wen;HUANG Yu-mei;MA Lin;LI Dong-dong;DOU De-qiang;LI Qin;Eucommia Ulmoides Cultivation and Utilization Laboratory of Henan Province;Institute of Chinese Materia Medica,Henan University;School of Pharmacy,Liaoning University of Traditional Chinese Medicine;

• 1:河南省杜仲栽培与利用工程实验室
• 2:河南大学中药研究所
• 3:辽宁中医药大学药学院

摘要(Abstract)：

该研究采用正相硅胶柱色谱,反相柱色谱(RP-18),凝胶色谱(Sephadex LH-20)以及重结晶等多种分离纯化手段,从杜仲雄花中分离得到9个化合物,通过化合物的理化性质和光谱数据鉴定其结构分别为:3-oxo-12-en-ursane-28-O-α-Larabinofuranosyl(1→6)-β-D-glucopyranoside(1),2α,3β-dihydroxyurs-12-en-28-oic acid(28→1)-β-D-glucopyranosyl ester(2),熊果酸(3),α-香树脂醇(4),熊果醇(5),3-O-乙酰基熊果酸乙酸酯(6),3-O-乙酰基齐墩果酸(7),白桦脂酸(8),白桦脂醇(9)。其中化合物1为新化合物,化合物2,4⁷为首次从该科植物中分离得到。采用MTT法对所分离的9个化合物进行抗肿瘤活性测试,结果表明仅有化合物3对K562和Hep G2细胞显示出一定的抗肿瘤活性,其余化合物对所选的2种细胞均无明显活性。
Nine triterpenes compounds were isolated from the male flowers of Eucommia ulmoides by recrystallization and chromatographic techniques over silica gel,Sephadex LH-20,and RP-18 gel. Their chemical structures were identified on the basis of spectral analysis and as 3-oxo-12-en-ursane-28-O-α-L-arabinofuranosyl(1 →6)-β-D-glucopyranoside(1),2α,3β-dihydroxyurs-12-en-28-oic acid(28→1)-β-D-glucopyranosyl ester(2),ursolic acid(3),α-amyrin(4),uvaol(5),ursolic acid acetate(6),3-O-acetate oleanoic acid(7),betulinic acid(8),and betulinol(9). Compound 1 was a new compound,and compounds 2,4-7 were isolated from the Eucommiu genus for the first time. Cytotoxic activity was tested for all the compounds against K562 and Hep G2 cells. The results showed that only compound 3,exhibited cytotoxic activity.

关键词(KeyWords)： 杜仲雄花;三萜;抗肿瘤活性
Eucommia ulmoides;triterpene;cytotoxic activity

Abstract:

Keywords:

基金项目(Foundation): 国家公益性行业专项基金项目(201004029)

作者(Authors): 丁艳霞;王腾宇;张耀文;黄玉梅;马琳;李冬冬;窦德强;李钦;
DING Yan-xia;WANG Teng-yu;ZHANG Yao-wen;HUANG Yu-mei;MA Lin;LI Dong-dong;DOU De-qiang;LI Qin;Eucommia Ulmoides Cultivation and Utilization Laboratory of Henan Province;Institute of Chinese Materia Medica,Henan University;School of Pharmacy,Liaoning University of Traditional Chinese Medicine;

参考文献(References)：

• [1]中国药典.一部[S].2010.
• [2]张康健,王蓝,马柏林,等.中国杜仲次生代谢物[M].北京:科学出版社,2002:168.
• [3]李竹,晏媛,李青.杜仲的药理活性研究进展[J].2004,18(2):131.
• [4]杜红岩,俞睿.杜仲产业绿皮书[M].北京:社会科学文献出版社,2012:354.
• [5]张秋萍,谢路现,邓涛,等.熊果酸促进K562细胞凋亡[J].基础医学与研究,2004,24(4):414.
• [6]张一斌.落新妇三萜酸E体外抗肿瘤活性剂及其作用机理研究[D].杭州:浙江大学,2007.
• [7]Dou Deqiang,Chen Yingjie,Ma Zhongze,et al.A novel minor saponin from the leaves of Panax ginseng.C.A.Meyer[J].J Chin Pharm Sci,1996,5(1):48.
• [8]A R Billa,E Palme,S Catalano.New triterpenoid saponins from the roots of Potentilla Tormentilla[J].J Nat Prod,1994,57(3):333.
• [9]Yin Hailong,Li Jian,Li Qingsheng,et al.Chemical constituents from Solanum lyratum Thunb[J].Bull Acad Mil Med Sci,2010,134(1):65.
• [10]莫小宇,麦景标.椿皮三氯甲烷部位化学成分研究(Ⅰ)[J].中国实验方剂学杂志,2012,18(21):103.
• [11]Reiko Fujita,Hongquan Duan,Yoshihisa Takaishi.Terpenoids from Tripterigyum hypoglaucum[J].Phytochemistry,2000,53:715.
• [12]林小菊,刘静涵,秦民坚,等.二球悬铃木树皮的化学成分[J].中国药科大学学报,2013,44(3):210.
• [13]高慧媛,吴立军,黑柳正典.光皮木瓜的化学成分[J].中国天然药物,2003,1(2):81.