中国中药杂志

2014, v.39(21) 4225-4229

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杜仲雄花中三萜类化学成分研究
Triterpenes constituents from male flowers of Eucommia ulmoides

丁艳霞;王腾宇;张耀文;黄玉梅;马琳;李冬冬;窦德强;李钦;
DING Yan-xia;WANG Teng-yu;ZHANG Yao-wen;HUANG Yu-mei;MA Lin;LI Dong-dong;DOU De-qiang;LI Qin;Eucommia Ulmoides Cultivation and Utilization Laboratory of Henan Province;Institute of Chinese Materia Medica,Henan University;School of Pharmacy,Liaoning University of Traditional Chinese Medicine;

摘要(Abstract):

该研究采用正相硅胶柱色谱,反相柱色谱(RP-18),凝胶色谱(Sephadex LH-20)以及重结晶等多种分离纯化手段,从杜仲雄花中分离得到9个化合物,通过化合物的理化性质和光谱数据鉴定其结构分别为:3-oxo-12-en-ursane-28-O-α-Larabinofuranosyl(1→6)-β-D-glucopyranoside(1),2α,3β-dihydroxyurs-12-en-28-oic acid(28→1)-β-D-glucopyranosyl ester(2),熊果酸(3),α-香树脂醇(4),熊果醇(5),3-O-乙酰基熊果酸乙酸酯(6),3-O-乙酰基齐墩果酸(7),白桦脂酸(8),白桦脂醇(9)。其中化合物1为新化合物,化合物2,47为首次从该科植物中分离得到。采用MTT法对所分离的9个化合物进行抗肿瘤活性测试,结果表明仅有化合物3对K562和Hep G2细胞显示出一定的抗肿瘤活性,其余化合物对所选的2种细胞均无明显活性。
Nine triterpenes compounds were isolated from the male flowers of Eucommia ulmoides by recrystallization and chromatographic techniques over silica gel,Sephadex LH-20,and RP-18 gel. Their chemical structures were identified on the basis of spectral analysis and as 3-oxo-12-en-ursane-28-O-α-L-arabinofuranosyl(1 →6)-β-D-glucopyranoside(1),2α,3β-dihydroxyurs-12-en-28-oic acid(28→1)-β-D-glucopyranosyl ester(2),ursolic acid(3),α-amyrin(4),uvaol(5),ursolic acid acetate(6),3-O-acetate oleanoic acid(7),betulinic acid(8),and betulinol(9). Compound 1 was a new compound,and compounds 2,4-7 were isolated from the Eucommiu genus for the first time. Cytotoxic activity was tested for all the compounds against K562 and Hep G2 cells. The results showed that only compound 3,exhibited cytotoxic activity.

关键词(KeyWords): 杜仲雄花;三萜;抗肿瘤活性
Eucommia ulmoides;triterpene;cytotoxic activity

Abstract:

Keywords:

基金项目(Foundation): 国家公益性行业专项基金项目(201004029)

作者(Authors): 丁艳霞;王腾宇;张耀文;黄玉梅;马琳;李冬冬;窦德强;李钦;
DING Yan-xia;WANG Teng-yu;ZHANG Yao-wen;HUANG Yu-mei;MA Lin;LI Dong-dong;DOU De-qiang;LI Qin;Eucommia Ulmoides Cultivation and Utilization Laboratory of Henan Province;Institute of Chinese Materia Medica,Henan University;School of Pharmacy,Liaoning University of Traditional Chinese Medicine;

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