中国中药杂志

2018, v.43(15) 3140-3144

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淫羊藿总黄酮胶囊中黄酮类成分含量测定及抗骨质疏松活性研究
Determination of flavonoids constituents in epimedium total flavone capsule and research on its anti-osteoporosis activity

徐忠坤;殷洪梅;李芳;秦建平;顾少莉;吴云;王振中;萧伟;
XU Zhong-kun;YIN Hong-mei;LI Fang;QIN Jian-ping;GU Shao-li;WU Yun;WANG Zhen-zhong;XIAO Wei;Jiangsu Kanion Pharmaceutical Co.,Ltd.;State Key Laboratory of Pharmaceutical Process New-tech for Chinese Medicine;Key Laboratory for the New Technique Research of Traditional Chinese Medicine Extraction and Purification;

摘要(Abstract):

采用快速液相色谱法(RRLC)对淫羊藿总黄酮胶囊进行含量测定,建立了淫羊藿新苷A、朝霍定A1、朝霍定A、朝霍定B、朝霍定C、淫羊藿苷、宝藿苷Ⅱ、淫羊藿次苷Ⅰ、箭藿苷B、鼠李糖基淫羊藿次苷Ⅱ、宝藿苷Ⅰ等11个黄酮类成分的含量测定方法。同时,通过检测碱性磷酸酶(AKP)含量,考察了11个化合物诱导MC3T3-E1细胞增殖分化的效果,结果显示宝藿苷Ⅱ活性最好,宝藿苷Ⅱ和淫羊藿次苷Ⅰ活性都大于淫羊藿苷。该研究建立了黄酮苷类成分的含量测定方法,并比较了每个单体抗骨质疏松的效果,为淫羊藿总黄酮胶囊药效物质基础和作用机制研究提供了技术支持。
To develop a rapid resolution liquid chromatography(RRLC) method for the simultaneous determination of epimedoside A,epimedin A1,epimedin A,epimedin B,epimedin C,icariin,baohuosideⅡ,icarisideⅠ,sagittatoside B,2″-O-rhamnosyl icarisideⅡ,and baohuosideⅠin epimedium total flavone capsule. At the same time,the effects of the above 11 compounds on osteogenic differentiation of MC3 T3-E1 cells were investigated by detecting the content of alkaline phosphatase(AKP). The results showed that baohuoside Ⅱ had the highest activities,and both the activities of baohuoside Ⅱ and icariside Ⅰ were stronger than those of icariin.In this study,the content determination method of flavonoid glycosides was established,and the anti-osteoporosis effect of monomers was compared,providing technical support for the study of the pharmacodynamic and mechanism of Epimedium total flavone capsule.

关键词(KeyWords): 淫羊藿总黄酮胶囊;黄酮苷;RRLC;MC3T3-E1;AKP
epimedium total flavone capsule;flavonoid glycoside;RRLC;MC3T3-E1;AKP

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基金项目(Foundation): 国家“重大新药创制”科技重大专项(2013ZX09402203)

作者(Author): 徐忠坤;殷洪梅;李芳;秦建平;顾少莉;吴云;王振中;萧伟;
XU Zhong-kun;YIN Hong-mei;LI Fang;QIN Jian-ping;GU Shao-li;WU Yun;WANG Zhen-zhong;XIAO Wei;Jiangsu Kanion Pharmaceutical Co.,Ltd.;State Key Laboratory of Pharmaceutical Process New-tech for Chinese Medicine;Key Laboratory for the New Technique Research of Traditional Chinese Medicine Extraction and Purification;

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