中国中药杂志

2018, v.43(03) 609-617

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外翻肠囊法研究羊耳菊提取物在大鼠肠内的吸收
Absorption of Inula cappa extract based on everted intestinal sac method

巩仔鹏;李梅;侯靖宇;吴林霖;陈亭亭;李月婷;陈思颖;李勇军;王爱民;兰燕宇;王永林;
GONG Zi-peng;LI Mei;HOU Jing-yu;WU Lin-lin;CHEN Ting-ting;LI Yue-ting;CHEN Si-ying;LI Yong-jun;WANG Ai-min;LAN Yan-yu;WANG Yong-lin;Guizhou Provincial Key Laboratory of Pharmaceutics,Guizhou Medical University;Engineering Research Center for the Development and Application of Ethnic Medicine and Traditional Chinese Medicine,Ministry of Education,Guizhou Medical University;School of Pharmacy,Guizhou Medical University;National Engineering Research Center of Miao's Medicines;

摘要(Abstract):

考察羊耳菊提取物在大鼠肠内的吸收特征,应用大鼠肠外翻模型,选择羊耳菊提取物中具有代表成分的东莨菪苷等9种成分为考察对象,建立其UPLC-MS/MS测定方法并测量其累计吸收量,阐明各成分在不同肠段的吸收特征。结果表明,不同浓度的1,3-O-二咖啡酰基奎宁酸等成分随活性部位提取物的给药剂量增加而增加,无明显高浓度饱和现象,提示其可能的吸收方式均为被动扩散,东莨菪苷同样在高浓度条件下存在饱和现象,提示可能的吸收方式为主动转运。绿原酸的主要吸收部位在十二指肠,1,3-O-二咖啡酰基奎宁酸、木犀草苷、3,4-O-二咖啡酰基奎宁酸和隐绿原酸的最佳吸收部位是空肠,东莨菪苷,新绿原酸,3,5-O-二咖啡酰基奎宁酸和4,5-O-二咖啡酰基奎宁酸的最佳吸收部位在回肠。以此推测,羊耳菊提取物中9种成分在小肠均有吸收,但各成分的吸收速率、最佳吸收部位及吸收机制不尽相同,提示小肠对羊耳菊提取物中9种成分的吸收具有选择性。
To investigate the absorptive characteristics of Inula cappa extract based on the rat everted intestinal sac method in vitro.Nine representative ingredients in I. cappa extract were selected as the study objects. An UPLC-MS/MS method was established to determine and detect their cumulative absorption amount for expounding the absorptive characteristics of ingredients in different intestinal sections. According to the results,the transport mechanism of 8 compounds showed passive diffusion by the reverted gut sac method.And scopolin was actively transported in the intestine. The best absorption site of chlorogenic acid was duodenum. The best absorption site of cryptochlorogenic acid,1,3-O-dicaffeoylquinic acid,luteolin-7-glucoside and 3,4-O-dicaffeoylquinic acid were jejunum. The best absorption site of neochlorogenic acid,scopolin,4,5-O-dicaffeoylquinic acid and 3,5-O-dicaffeoylquinic acid was ileum. The absorption of all the compounds was affected by p H and bile. All of the nine ingredients in I. cappa extract could be absorbed in intestines,but with differences in the absorption rate,the best absorptive site and mechanism,indicating that the intestinal absorption of I. cappa extract was selective.

关键词(KeyWords): 羊耳菊提取物;咖啡酰基奎宁酸;吸收;肠外翻模型
Inula cappa extract;dicaffeoylquinic acid;absorption;everted intestinal sac method

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81360680);; 贵州省优秀青年科技人才培养项目(黔科合人字[2015]11号);; 贵州省民族药药效物质基础研究科技创新人才团队/贵州省高层次创新型人才培养项目(百层次黔科合平台人才[2016]5613/5677);; 贵州省教育厅项目(黔科合KY字[2013]122);; 贵州省科技合作计划项目(黔科合LH字[2015]7362)

作者(Author): 巩仔鹏;李梅;侯靖宇;吴林霖;陈亭亭;李月婷;陈思颖;李勇军;王爱民;兰燕宇;王永林;
GONG Zi-peng;LI Mei;HOU Jing-yu;WU Lin-lin;CHEN Ting-ting;LI Yue-ting;CHEN Si-ying;LI Yong-jun;WANG Ai-min;LAN Yan-yu;WANG Yong-lin;Guizhou Provincial Key Laboratory of Pharmaceutics,Guizhou Medical University;Engineering Research Center for the Development and Application of Ethnic Medicine and Traditional Chinese Medicine,Ministry of Education,Guizhou Medical University;School of Pharmacy,Guizhou Medical University;National Engineering Research Center of Miao's Medicines;

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