中国中药杂志

2017, v.42(12) 2361-2365

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冬凌草甲素诱导三阴乳腺癌MDA-MB-231细胞凋亡及对细胞内活性氧水平的影响
Effect of oridonin on apoptosis and intracellular reactive oxygen species level in triple-negative breast cancer MDA-MB-231 cells

齐琦;张配;李其响;潘琼;郑海伦;赵素容;
QI Qi;ZHANG Pei;LI Qi-xiang;PAN Qiong;ZHENG Hai-lun;ZHAO Su-rong;Department of Basic Medical Sciences,Bengbu Medical College;School of Pharmacy,Bengbu Medical College;First Affiliated Hospital of Bengbu Medical College;

摘要(Abstract):

冬凌草甲素是从中药冬凌草Rabdosia rubescens中分离得到的一种贝壳杉烯二萜类的天然有机化合物,具有抗炎、抗菌、抗肿瘤等多种生物活性。该实验观察冬凌草甲素对三阴乳腺癌MDA-MB-231细胞凋亡的影响及其可能的作用机制。采用MTT法检测冬凌草甲素对MDA-MB-231细胞的增殖抑制作用,PI单染、Annexin V-FITC/PI双染流式细胞术分析冬凌草甲素对MDA-MB-231细胞凋亡的影响,活性氧(ROS)试剂盒检测细胞内ROS水平的变化,Western blot分析PARP,Bcl-2,caspase-3蛋白的表达情况。结果表明,冬凌草甲素对乳腺癌MDA-MB-231细胞具有明显的凋亡诱导作用,显著升高细胞内的ROS水平,下调抗凋亡蛋白Bcl-2的表达,并促进caspase-3及其底物PARP的切割。提示,冬凌草甲素诱导MDA-MB-231细胞凋亡的作用可能与升高细胞内ROS水平、下调Bcl-2的表达以及激活caspase-3相关。
Oridonin,which is an ent-kaurene diterpenoid isolated from traditional Chinese medicine Rabdosia rubescens,displays various bioactivities,including anti-inflammation,anti-bacteria and anti-tumor.This study aimed to investigate the effect of oridonin on apoptosis of triple-negative breast cancer MDA-MB-231 cells and its underlying mechanisms.The inhibitory effect of oridonin on proliferation of MDA-MB-231 cells was measured by MTT assay;Apoptosis was analyzed by flow cytometry with PI staining and Annexin VFITC/PI staining;Intracellular reactive oxygen species(ROS) level was determined by ROS detection kit,and expressions of PARP,Bcl-2,caspase-3 were analyzed by Western blot.The results showed that oridonin exhibited a significant effect in inducing apoptosis of MDA-MB-231 cells,enhancing intracellular ROS level,down-regulating expression of Bcl-2 protein,and promoting cleavage of caspase-3 and its substrate PARP.These results indicated that the apoptosis-inducing effect of oridonin on MDA-MB-231 cells might be correlated with increase of intracellular ROS level,down-regulation of Bcl-2 protein and activation of caspase-3.

关键词(KeyWords): 乳腺癌;冬凌草甲素;细胞凋亡;活性氧
breast cancer;oridonin;apoptosis;reactive oxygen

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81372899,81603155);; 安徽省高等学校自然科学研究重点项目(KJ2016A486,KJ2016A869,KJ2015A177)

作者(Authors): 齐琦;张配;李其响;潘琼;郑海伦;赵素容;
QI Qi;ZHANG Pei;LI Qi-xiang;PAN Qiong;ZHENG Hai-lun;ZHAO Su-rong;Department of Basic Medical Sciences,Bengbu Medical College;School of Pharmacy,Bengbu Medical College;First Affiliated Hospital of Bengbu Medical College;

DOI: 10.19540/j.cnki.cjcmm.2017.0114

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