中国中药杂志

2009, v.34(18) 2305-2307

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半夏内生菌的分离与初步鉴定
Isolation and identification of endophytes from Pinellia

刘建玲;陈宝宝;刘永红;郑姜彬;梁宗锁;
LIU Jianling1*,CHEN Baobao1,LIU Yonghong2,ZHENG Jiangbin1,LIANG Zongsuo2(1.College of Life Sciences,Northwest University,Xi'an 710069,China;2.College of Life Sciences,Northwest Agriculture and Forestry University,Yangling 712100,China)

摘要(Abstract):

目的:探讨半夏内生菌类群的多样性。方法:采用平板分离法分离半夏的内生真菌、细菌、放线菌,同时采用点植法、插片法和印片法对分离真菌菌株进行分类鉴定。结果:从多年生和一年生的半夏根茎叶样品中共获得内生菌84株,其中内生真菌48株、细菌31株、放线菌5株,内生真菌经形态观察分类鉴定为4科、15属。结论:半夏内生菌多样性丰富,同时不同部位内生菌的数量、种类及分布存在差异。
Objective: To investigate the varieties of entophytes in different parts of Pinellia ternata.Method: The solidified plates were applied for the isolation of the endophytes,and three methods were used for the identification of endophytic fungi.Result: Eighty four strains of the entophytes were isolated from the P.ternata collected from 3 habitations.Endophytic fungi were morphologically identified belonging to 15 genera,4 families.Conclusion: It indicated that the entophytes in P.ternata were diversity and rich,and there were some differences at quantity and species in different organs of P.ternata.

关键词(KeyWords): 半夏;内生菌;分离鉴定
Pinellia ternata;endophyte;isolation and identification

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基金项目(Foundation): 教育部新世纪优秀人才支持计划项目;; 西北农林科技大学学科带头人支持计划项目

作者(Author): 刘建玲;陈宝宝;刘永红;郑姜彬;梁宗锁;
LIU Jianling1*,CHEN Baobao1,LIU Yonghong2,ZHENG Jiangbin1,LIANG Zongsuo2(1.College of Life Sciences,Northwest University,Xi'an 710069,China;2.College of Life Sciences,Northwest Agriculture and Forestry University,Yangling 712100,China)

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