中国中药杂志

2021, v.46(24) 6312-6322

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柬埔寨金边乌亚希草药市场草药分子鉴定及功效分析
Molecular identification and efficacy analysis of herbs at Orussey Herbal Market, Phnom Penh, Cambodia

魏雪苹;董雨青;强亭燕;李文杰;宋奕辰;张本刚;张昭;HUOT Theang;刘海涛;齐耀东;
WEI Xue-ping;DONG Yu-qing;QIANG Ting-yan;LI Wen-jie;SONG Yi-chen;ZHANG Ben-gang;ZHANG Zhao;HUOT Theang;LIU Hai-tao;QI Yao-dong;Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education,Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College;College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry University;National Centre for Traditional Medicine;

摘要(Abstract):

柬埔寨具有丰富的药用植物资源,在柬埔寨金边乌亚希草药市场收集植物类药材133种,药材类型包括全草、根、茎/枝、叶、花、果实、种子、树脂等。利用ITS和psbA-trnH对以上药材进行了鉴定,其中ITS序列共鉴定出46个样品,分别属于24科40属42种,psbA-trnH序列共鉴定出100个样品,分别属于42科77属84种,通过2种DNA条形码共鉴定出了103个样品。结合药材性状特征对鉴定结果进行了复查和核对,最终成功鉴定出120个样品,分别属于50科86属86种,数目最多的为姜科、豆科、爵床科药材。其中有比较多的药材是柬埔寨传统医药、阿育吠陀医药、尤纳尼医药以及我国中医药和民族药的习用药材,但同种药材在不同医药体系中所侧重的功效存在差异。该研究结果表明DNA条形码在鉴定草药产品方面具有显著优势,同时该研究为了解柬埔寨习用药材提供了基础数据。
Cambodia is rich in medicinal plant resources. One hundred and thirty-three medicinal material samples, including the hole herb, root, stem/branch, leaf, flower, fruit, seed, and resin, were collected from the Orussey Herbal Market in Phnom Penh, Cambodia, and then authenticated by ITS and psbA-trnH. A total of 46 samples were identified based on ITS sequences, belonging to 24 families, 40 genera, and 42 species. A total of 100 samples were identified by psbA-trnH sequences to belong to 42 families, 77 genera, and 84 species. A total of 103 samples were identified by two DNA barcodes. According to the morphological characteristics of the medicinal materials, 120 samples classified into 50 species, 86 genera, and 86 families were identified, and the majority of them were from Zingiberaceae, Fabaceae, and Acanthaceae. Such samples have been commonly used in traditional Cambodian medicine, Ayurvedic medicine, Unani medicine, traditional Chinese medicine, and ethnomedicine, but different medical systems focus on different functional aspects of the same medicinal material. The results of this study have demonstrated that DNA barcoding has a significant advantage in identifying herbal products, and this study has provided basic data for understanding the traditional medicinal materials used in Cambodia.

关键词(KeyWords): 柬埔寨;草药;民族药;DNA条形码;分子鉴定
Cambodia;herbs;ethno medicine;DNA barcodes;molecular identification

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基金项目(Foundation): 科技部高端外国专家引进计划项目(G20190001659);科技部科技基础资源调查专项(2018FY100703);; 中国医学科学院医学与健康科技创新工程项目(2017-I2M-B&R-09)

作者(Author): 魏雪苹;董雨青;强亭燕;李文杰;宋奕辰;张本刚;张昭;HUOT Theang;刘海涛;齐耀东;
WEI Xue-ping;DONG Yu-qing;QIANG Ting-yan;LI Wen-jie;SONG Yi-chen;ZHANG Ben-gang;ZHANG Zhao;HUOT Theang;LIU Hai-tao;QI Yao-dong;Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education,Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College;College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry University;National Centre for Traditional Medicine;

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