中国中药杂志

2013, v.38(20) 3424-3431

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胞内、外Ca~(2+)在水杨酸诱导丹参幼苗迷迭香酸生物合成过程中的作用
Regulation effects of intracellular and extracellular Ca~(2+) on biosynthesis of rosmarinic acid induced by salicylic acid in young seedlings of Salvia miltiorrhiza

曹蓉蓉;党小琳;行冰玉;张婧一;董娟娥;
CAO Rong-rong;DANG Xiao-lin;XING Bing-yu;ZHANG Jing-yi;DONG Juan-e;College of Life Sciences,Northwest Agricultural and Forestry University;

摘要(Abstract):

目的:为探讨胞内、外钙离子是否在水杨酸诱导丹参迷迭香酸生物合成中起作用。方法:以生长50 d的丹参幼苗为材料,通过喷施水杨酸诱导迷迭香酸合成量增加,再利用胞外钙通道抑制剂verapamil(Vp)和LaCl3、胞内钙调素拮抗剂trifluoperazine(TFP)和胞内钙通道上IP3受体抑制剂LiCl处理,分别考察水杨酸、钙离子通道抑制剂/钙调素拮抗剂处理后迷迭香酸的合成量及其合成相关酶(PAL,TAT)的活性变化。结果:2.0 mmol·L-1的水杨酸处理24 h后可诱导迷迭香酸合成量升高到(40.51±2.16)mg·g-1,是对照的1.97倍,迷迭香酸合成相关酶PAL活性升高到对照的1.42倍,TAT活性升高到对照的1.29倍;Vp,LaCl3,TFP,LiCl处理均抑制了水杨酸诱导的PAL,TAT活性的升高,从而导致了迷迭香酸合成量降低。结论:水杨酸诱导丹参迷迭香酸生物合成过程中,胞内、外钙离子发挥着重要的信号转导作用。
Objective: To investigate the effect of intracellular and extracellular Ca2 +on the biosynthesis of rosmarinic acid( RA) induced by salicylic acid in young seedlings of Salvia miltiorrhiza. Method: Young seedlings of S. miltiorrhiza were used to select an optimal concentration of salicylic acid( SA),and then use the optimal concentration of SA to investigate the effects of extracellular Ca2 +channel inhibitors Verapamil,LaCl 3,intracelluar calmodulin antagonist TFP and intracelluar Ca2 +channel inhibitors LiCl on the biosynthesis of RA and related enzymes. Result: SA increased the accumulation of RA and the activities of PAL and TAT,especially the SA of 2 mmol·L- 1after 24 h. SA improved the accumulation of RA to( 40. 51 ± 2. 16) mg·g- 1,which was 1. 97 times than that of control,and the activities of PAL,TAT were 1. 42 times and 1. 29 times than those of the control. However,Vp,LaCl 3, TFP,LiCl inhibited the effects of SA evidently. Conclusion: Ca2 +plays a key role in the regulation of the induction process.

关键词(KeyWords): 丹参;水杨酸;PAL和TAT;迷迭香酸;钙通道抑制剂;钙调素拮抗剂
Salvia miltiorrhiza;salicylic acid;PAL and TAT;rosmarinic acid;Ca2 +channel blockers;calmodulin antagonist

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(31170274);; 西北农林科技大学青年骨干支持计划项目

作者(Author): 曹蓉蓉;党小琳;行冰玉;张婧一;董娟娥;
CAO Rong-rong;DANG Xiao-lin;XING Bing-yu;ZHANG Jing-yi;DONG Juan-e;College of Life Sciences,Northwest Agricultural and Forestry University;

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