中国中药杂志

2018, v.43(10) 2112-2117

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基于抗凝血酶活性效价的麝香质量生物评价方法研究
Quality evaluation of musk based on the biological potency of its anti-thrombin activty

罗云;谭婷;梁新丽;赵海平;廖正根;杨明;
LUO Yun;TAN Ting;LIANG Xin-li;ZHAO Hai-ping;LIAO Zheng-gen;YANG Ming;Key Laboratory of Modern Preparation of Ttaditional Chinese Medicine,Ministry of Education,Jiangxi University of Traditional Chinese Medicine;National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herbal Medicine,Jiangxi University of Traditional Chinese Medicine;

摘要(Abstract):

该文尝试建立麝香体外抗凝血酶活性效价测定方法,探索品种和产地对麝香抗凝血酶生物活性的影响,为麝香质量生物评价提供科学依据。采用凝血酶滴定法,考察麝香供试品溶液浓度、纤维蛋白原浓度、凝血酶浓度、凝血酶滴定时间间隔对麝香抗凝血酶活性测定的影响,并比较不同品种和产地麝香抗凝血酶活性的大小。结果显示,麝香供试品溶液浓度在一定范围内(0.01~0.02 g·m L~(-1)),纤维蛋白原浓度为0.5%,凝血酶浓度为10 U·m L~(-1),滴定间隔时间为1 min,每次滴定2μL时,麝香供试品溶液浓度与凝血酶消耗体积呈良好的线性关系(r=0.991 4)。不同产地林麝麝香抗凝血酶活性效价为(105.0±10.4)U·g~(-1),马麝麝香抗凝血酶活性效价为(102.4±5.5)U·g~(-1),原麝(安徽)麝香抗凝血酶活性效价为(97.7±6.6)U·g~(-1),人工麝香抗凝血酶活性效价为(58.6±6.4)U·g~(-1)。结果表明,抗凝血酶活性效价测定方法简便、快速、准确、可靠,可用于麝香质量生物评价;品种和产地对麝香抗凝血酶活性影响较大,在麝人工饲养时应注意品种和产地的选择;人工麝香和天然麝香的抗凝血酶活性差异较大,提示人工麝香处方配比可能有待进一步优化。
This study attempts to establish a method for the anti-thrombin activty bioassay of musk,explore the impact of species and producing areas on the anti-thrombin activty of musk,and provide scientific basis for its biological quality evaluation. Anti-thrombin activty of musk was analyzed by thrombin titration,and the influence factors such as musk solution concentration,fibrinogen concentration,thrombin concentration and titration interval were optimized to evaluate the effect of different species and producing areas on anti-thrombin activty of musk. As a result,there was a good linear relationship between musk solution concentration and thrombin consumption volume within the range of 0. 01-0. 02 g·m L~(-1)( r = 0. 991 4) under the experimental condition as follows: fibrinogen concentration was 0. 5%,the thrombin concentration was 10 U·m L~(-1); titration time interval was once every minute,and each titration volume was 2 μL. The average anti-thrombin activty potency of Moschus berezovskii from different producing areas was( 105. 0 ± 10. 4) U·g~(-1),( 102. 4 ± 5. 5) U·g~(-1) for M. sifanicus from different producing areas,( 97. 7 ± 6. 6) U·g~(-1) for M. moschiferus from Anhui province,and( 58. 6 ± 6. 4) U·g~(-1) for artificial musk. The results indicated that this anti-thrombin activty bioassay method could be applied to evaluate the anti-thrombin activty of musk quickly,conveniently,sensitively and exactly. It was also suggested that different species and producing areas had effects on the anti-thrombin activty of musk,so it is necessary to pay attention to species and producing areas in the process of musk domestication; meanwhile,the artificial musk and natural musk also showed great differences in anti-thrombin activty,suggesting that ratio in artificial musk prescription needs to be further optimized.

关键词(KeyWords): 麝香;抗凝血酶活性;凝血酶滴定法;产地;品种;生物测定
musk;anti-thrombin activty;thrombin titration;species;producing area;bioassay

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81460600,81603274,81760711);; 江西省自然科学基金项目(20171BAB205100,20161BAB215189);; 江西省卫生计生委中医药科研项目(2016A042);; 江西中医药大学院士工作站专项(2016ZR001)

作者(Author): 罗云;谭婷;梁新丽;赵海平;廖正根;杨明;
LUO Yun;TAN Ting;LIANG Xin-li;ZHAO Hai-ping;LIAO Zheng-gen;YANG Ming;Key Laboratory of Modern Preparation of Ttaditional Chinese Medicine,Ministry of Education,Jiangxi University of Traditional Chinese Medicine;National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herbal Medicine,Jiangxi University of Traditional Chinese Medicine;

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