中国中药杂志

2014, v.39(12) 2311-2313

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木通皂苷D在大鼠胃肠道内容物中的稳定性考察
Stability of akebia saponin D in gastrointestinal contents of rats

宫灿仪;萧伟;王振中;杨中林;
GONG Can-yi;XIAO Wei;WANG Zhen-zhong;YANG Zhon-glin;State Key Laboratory of Natural Medicines-China Pharmaceutical University;Kanion pharmaceutical;

摘要(Abstract):

为了考察木通皂苷D(akebia saponin D,ASD)在胃肠道内容物中的稳定性,建立了ASD的高效液相色谱含量测定方法,并将ASD分别溶解在人工胃液、人工肠液、胃内容物、大肠内容物、小肠内容物中,37℃恒温水浴,孵育一定时间后取样,测定ASD的浓度。结果 ASD在大肠内容物中极不稳定,8 h以内被全部降解。ASD在人工胃液、人工肠液、胃内容物和小肠内容物中非常稳定。上述结果证明ASD在酸性环境下、酶的存在下以及在小肠菌群中均稳定,而在大肠菌群的作用下极易降解。
In order to study the stability of akebia saponin D(ASD) in biological fluids in vitro,the determination methods of ASD were established in this study. Akebia saponin D was dissolved in artificial gastric juice,intestinal juice and gastrointestinal contents of rats,respectively,then thermostatically maintained at 37 ℃. At time intervals after degradation,samples were withdrawn and the concentrations of ASD were determined by HPLC,from which stability of it at different biological specimen was evaluated. As a result,ASD was totally degraded in large intestinal contents of rats in 8 hours. ASD was very stable in artificial gastric juice,intestinal juice and gastric contents of rats. All of the above data proved that ASD was easily degraded by coliform bacteria but stable in acid environment and with the presence of digestive enzyme.

关键词(KeyWords): 木通皂苷D;稳定性;高效液相色谱法
akebia saponin D;HPLC;stability

Abstract:

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基金项目(Foundation): 国家“重大新药创制”科技重大专项(2009ZX09102-118);; 江苏省优势学科支持项目

作者(Author): 宫灿仪;萧伟;王振中;杨中林;
GONG Can-yi;XIAO Wei;WANG Zhen-zhong;YANG Zhon-glin;State Key Laboratory of Natural Medicines-China Pharmaceutical University;Kanion pharmaceutical;

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