中国中药杂志

2008, (02) 136-139

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

大孔吸附树脂分离富集分离葶苈子中总强心苷
Enrichment of total cardiac glycoside from Semen Descuraniae by macroporous adsorption resin

祁琴;石晋丽;闫兴丽;万新;王爱芹;王秀坤;
QI Qin1,SHI Jin-li1,YAN Xing-li1,WAN Xin1,WANG Ai-qin2,WANG Xiu-kun1(1.Beijing University of Chinese Medicine,Beijing 100102,China;2.Xuan wu No3 Vocational School in Beijing,Beijing 100053,China)

摘要(Abstract):

目的:考察6种大孔树脂对葶苈子总强心苷的分离富集分离性能,确定最佳工艺条件。方法:以总强心苷吸附量,洗脱率,洗脱物总强心苷含量为指标,采用紫外-可见分光光度法进行含量测定。结果:NKA-9型树脂对葶苈子中总强心苷具有较好的分离富集分离能力,其最佳的工艺条件为:葶苈子提取物质量浓度4 g.mL-1(生药量),最大吸附量3.54 mg.g-1,装柱径高比1/3,吸附流速2 mL.m in-1,水洗脱量3倍柱体积,洗脱剂80%乙醇,洗脱量3倍柱体积,洗脱速度1 mL.m in-1。结论:NKA-9型树脂分离富集总强心苷的最佳工艺稳定高效,可推广应用于生产。
Objective: To establish the separation and enrichment technology for total cardiac glycoside from Semen descuraniae,6 types of macroporous adsorption resin were investigated.Method: The adsorption capacity,elution ratio and purify of total cardiac glycoside were used as evaluating criteria.The UV-VIS spectrophotometric method was used in the determination of total cardiac glycoside content.Result: NKA-9 had better property of separation and purification and the optimal technological conditions were: the concentration of the sample of Semen Descuraniae,4 g·mL-1(amount to crude drug),The maximum adsorption capacity of total cardiac glycoside,3.54 mg·g-1,the optimal column diameter/height,1/3,the flow velocity of adsorption,2 mL·min-1,washing water,3 times of resin volume,the eluting reagent,80% ethanol(3 times of the resin volume),eluting velocity,1 mL·min-1.Conclusion: The technology of separating and purifying total cardiac glycoside of Semen Descuraniae with NKA-9 was stable and effective,and can be applied to manufacture.

关键词(KeyWords): 葶苈子;总强心苷;大孔吸附树脂
Semen Descuraniae;total cardiac glycoside;macroporous adsorption resin

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(30572463)

作者(Author): 祁琴;石晋丽;闫兴丽;万新;王爱芹;王秀坤;
QI Qin1,SHI Jin-li1,YAN Xing-li1,WAN Xin1,WANG Ai-qin2,WANG Xiu-kun1(1.Beijing University of Chinese Medicine,Beijing 100102,China;2.Xuan wu No3 Vocational School in Beijing,Beijing 100053,China)

Email:

DOI:

扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享