中国中药杂志

2003, (04)

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灵芝醇溶酸性组分的制备工艺与检测方法研究
Study on Preparation Process and Analytical Methods of ESAC from Ganoderma lucidum

黄书铭,杨新林,王帮武,朱鹤孙,徐建兰
HUANG Shu ming 1, YANG Xin lin 1, WANG Bang wu 1, ZHU He sun 1, XU Jian lan 2(1. School of Life Sciences and Technology, Beijing Institute of Technology, Beijing 100081, China; 2. Wuxi Sanlian High tech Development Co. Ltd., Wuxi 214023,Jiangsu, China)

摘要(Abstract):

目的 :研究灵芝子实体中醇溶酸性组分 (ethanol solubleandacidiccomponents ,ESAC)制备工艺 ,并建立一种定量测定EASC含量的方法。方法 :灵芝精粉用无水乙醇浸泡 ,碱提酸化和氯仿萃取得ESAC组分 ;利用ESAC与浓硫酸的特征性颜色反应进行定量测定。结果 :1 0 g级ESAC较优的制备工艺为 :灵芝精粉与无水乙醇的投料比 1 (g)∶1 5(mL) ,浸提时间 2 4h ,饱和NaHCO3溶液和氯仿萃取量分别为 1 30 0mL ,分 3次萃取。ESAC定量测定条件为 :样品量 1g ,溶剂 1 5mL ,浸泡 0 .5h ,50 %H2 SO4 乙醇溶液 ,1 0 0℃水浴加热 3min ,测定 490nm处光吸收值。结论 :本文提供的ESAC制备的工艺路线 ,具有设计合理、操作简便的特点 ,建立的ESAC含量的测定方法可以用于灵芝相关产品的质量鉴定
Objective: To develop the procedure for separating the ethanol soluble and acidic components (ESAC) from Ganoderma lucidum , and to establish a method for quantifying ESAC in G. lucidum. Method: The ethanol extract of G. lucidum was extracted with a saturated NaHCO 3 solution, acidified and re extracted by chloroform to obtain ESAC. The quantitative analysis of ESAC was based on the characteristic color reaction between ESAC and H 2SO 4. Result: The optimal conditions for separating ESAC on a 10 g scale are as follows: ratio of material and ethanol (mL), 1∶ 15; immersing time, 24 h; volume of saturated NaHCO 3 and chloroform, 1300 mL; extract 3 times. The condition for measuring ESAC is as follows: sample weight, 1 g; solution volume, 1.5 mL; immuersing time, 0.5 h; detecting reagent, 50% H 2SO 4 in ethanol; heating time in 100 ℃ water bathe, 3 min; measuring wavelength, 490 nm. Conclusion: The procedure for ESAC preparation is simple and well designed, and the established method for ESAC can be used for the qualitative analysis of the G.lucidum related products. [

关键词(KeyWords): 灵芝;醇溶酸性组分;制备;测定
G. lucidum; ethanol soluble and acidic components (ESAC) ;preparation; measurement

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基金项目(Foundation): 国家重点科技项目 (攻关 )计划;; 原国家科委生命科学技术发展中心主任基金项目

作者(Author): 黄书铭,杨新林,王帮武,朱鹤孙,徐建兰
HUANG Shu ming 1, YANG Xin lin 1, WANG Bang wu 1, ZHU He sun 1, XU Jian lan 2(1. School of Life Sciences and Technology, Beijing Institute of Technology, Beijing 100081, China; 2. Wuxi Sanlian High tech Development Co. Ltd., Wuxi 214023,Jiangsu, China)

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