中国中药杂志

2016, v.41(20) 3798-3804

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基于高分离度和高色谱峰纯度的红参UPLC指纹图谱研究
Study on UPLC fingerprint of red ginseng based on good separation and good purity of chromatographic peaks

冯伟红;李春;吉丽娜;杨立新;荣立新;陈两绵;易红;王智民;
FENG Wei-hong;LI Chun;JI Li-na;YANG Li-xin;RONG Li-xin;CHEN Liang-mian;YI Hong;WANG Zhi-min;Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences;National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines;Tianjin Institute for Drug Control;Guang'anmen Hospital,China Academy of Chinese Medical Sciences;

摘要(Abstract):

采用UPLC建立红参指纹图谱。采用Waters Acquity BEH C18(2.1 mm×50 mm,1.7μm)色谱柱进行分离,乙腈-水为流动相,梯度洗脱,检测波长203 nm。通过对22批红参进行测定,建立了红参的UPLC指纹图谱,并定义了26个共有峰。采用对照品比对,指认了其中的11个共有峰,它们分别是人参皂苷Rg_1、人参皂苷Re、人参皂苷Rf、人参皂苷Rh1、人参皂苷Rg_2、人参皂苷Rb_1、20(S)-人参皂苷F1、人参皂苷Rb_2、人参皂苷Rb3、20(S)-人参皂苷Rg_3、20(R)-人参皂苷Rg_3。其中的20(S)-人参皂苷Rg_3和20(R)-人参皂苷Rg_3这一组差向异构体是红参的特征成分,它们不仅可以用于区分红参与人参,同时也可用于红参炮制的过程控制。采用"中药色谱指纹图谱相似度评价系统"对22批红参UPLC指纹图谱进行了相似度评价,结果 18批样品的相似度大于0.9。与文献报道的红参指纹图谱测定方法比较,该研究所建立的方法具有高效、专属性强、分离度高、色谱峰纯度高、方法简单易行的特点,可用于人参类药材的品种鉴定和质量控制,并为上述药材质量标准的提升提供了理论依据。
This study is to establish the UPLC fingerprint of red ginseng. The separation was performed on a Waters Acquity BEH C18column( 2. 1 mm × 50 mm,1. 7 μm),with the mobile phase consisting of acetonitrile and water for gradient elution. The detection wavelength was set at 203 nm. The UPLC fingerprint of red ginseng was established by using sample chromatography of 22 different purchase areas and 26 common peaks were found. Compared with the reference substances,11 of the common peaks were identified as ginsenosides Rg_1,ginsenoside Re,ginsenoside Rf,ginsenoside Rh_1,ginsenoside Rg_2,ginsenoside Rb1,20( S)-ginsenoside F1,ginsenoside Rb_2,ginsenoside Rb_3,20( S)-ginsenoside Rg_3 and 20( R)-ginsenoside Rg_3,respectively. It is worth noting that 20( S)-ginsenoside Rg_3 and 20( R)-ginsenoside Rg_3 are the characteristic ingredients of red ginseng,and they could be used not only for distinguishing red ginseng and ginseng,but also for process controlling of the preparation of red ginseng. The similarity was analyzed with'Similarity Evaluation System for Chromatographic Fingerprint of Chinese Materia Medica,and the similarity of 18 batches samples is up to 0. 9. Compared to the literature methods,the method is simple,time-saving,specific for the separation of ginsenosides from red ginseng. So,this method could be used for the species identification and quality control of ginseng,red ginseng and American ginseng,and it will alsoprovide a theoretical basis of raising quality standards of the above mentioned Chinese herb medicines.

关键词(KeyWords): UPLC;红参;人参皂苷;指纹图谱;高分离度;高色谱峰纯度
UPLC;red ginseng;ginsenosides;fingerprint chromatogram;good separation;good purity

Abstract:

Keywords:

基金项目(Foundation): 国家药品标准提高暨2015版药典科研任务;; 中药质量标准研究和信息化体系建设平台项目(2012ZX09304005);; 国家“重大新药创制”科技重大专项(2014ZX09304307001-002-007)

作者(Author): 冯伟红;李春;吉丽娜;杨立新;荣立新;陈两绵;易红;王智民;
FENG Wei-hong;LI Chun;JI Li-na;YANG Li-xin;RONG Li-xin;CHEN Liang-mian;YI Hong;WANG Zhi-min;Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences;National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines;Tianjin Institute for Drug Control;Guang'anmen Hospital,China Academy of Chinese Medical Sciences;

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