郭尚鑫;邵静媛;王毅;瞿海斌;龚行楚;
GUO Shang-xin;SHAO Jing-yuan;WANG Yi;QU Hai-bin;GONG Xing-chu;Pharmaceutical Informatics Institute,College of Pharmaceutical Sciences,Zhejiang University;

• 1:浙江大学药学院药物信息学研究所

摘要(Abstract)：

该研究使用纸基芯片技术测定丹红注射液制剂及中间体抗氧化活性。首先采用3D打印机把聚己内酯打印在滤纸表面,然后以聚己内酯和石蜡为改性剂制作了改性滤纸,接着在滤纸上加入DPPH乙醇溶液制成纸基芯片。以不同浓度抗坏血酸溶液为阳性药在纸基芯片上开展颜色反应,然后将纸基芯片烘干,并用智能手机采集数据。根据拟合所得决定系数大小、检测限高低以及测量值的有效位数筛选出灰度和颜色分量G作为备选检测指标。以丹红注射液制剂和水提浓缩液为对象,采用纸基芯片实现了抗氧化活性的定性和定量分析。由于2种备选检测指标所得抗氧化活性值之间无统计学差异,所以该研究将它们的结果取平均,计算得到丹红注射液和水提浓缩液的抗氧化活性分别相当于3.7,46 g·L-1的抗坏血酸溶液。该研究中提出的纸基芯片检验技术为中药制剂与中间体的生物活性评价提供了一种简便的方法。
The paper-based analytical device( PAD) was applied in this study to analyze the antioxidant activity of Danhong injection and its intermediates. First polycaprolactone was printed on the surface of a filter paper with a 3 D printing device. The modified filter paper was then prepared using polycaprolactone and solid paraffin as the modifiers. The PAD was prepared after adding DPPH ethanol solution to the modified filter paper. Ascorbic acid solutions with different concentrations were used as the positive drug on PAD. After the occurrence of color reactions,the PAD was dried,and the data of color were collected by a cell phone. The color component G and grayscale were selected as the potential indices for measurement according to the values of determination coefficients,detection limits,and effective number of digits. Qualitative and quantitative analysis of Danhong injection and the concentrate of aqueous extract were realized with the PAD. Because no significant differences were observed between the results obtained using the two potential indices,the average values of these two were used for analysis,and the antioxidant activity of Danhong injection and the concentrate of aqueous extract was equivalent to ascorbic acid solutions of 3. 7,46 g·L-1,respectively. The PAD method presented in this work can be a simple method to determine biological activities of Chinese medicines and their intermediates.

关键词(KeyWords)： 纸基芯片;丹红注射液;颜色分量;3D打印;质量控制
paper-based analytical devices;Danhong injection;color component;3D printing;quality control

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金青年基金项目(81503242)

作者(Author): 郭尚鑫;邵静媛;王毅;瞿海斌;龚行楚;
GUO Shang-xin;SHAO Jing-yuan;WANG Yi;QU Hai-bin;GONG Xing-chu;Pharmaceutical Informatics Institute,College of Pharmaceutical Sciences,Zhejiang University;

Email:

DOI: 10.19540/j.cnki.cjcmm.20180208.007

参考文献(References)：

• [1]齐云龙,丁永胜.图解纸芯片制作及应用进展[J].现代仪器与医疗,2013(3):7.
• [2]Cao R,Tian W,Shen W.Polysaccharides as protectants for paper-based analytical devices with antibody[J].Talanta,2017,165(C):357.
• [3]Meelapsom R,Jarujamrus P,Amatatongchai M,et al.Chromatic analysis by monitoring unmodified silver nanoparticles reduction on double layer microfluidic paper-based analytical devices for selective and sensitive determination of mercury(Ⅱ)[J].Talanta,2016,155(C):193.
• [4]Kappi F A,Tsogas G Z,Christodouleas D C,et al.Calibrantloaded paper-based analytical devices for standard addition quantitative assays[J].Sens Actuat B Chem,2017,253(C):860.
• [5]Songjaroen T,Dungchai W,Chailapakul O,et al.Blood separation on microfluidic paper-based analytical devices[J].Lab Chip,2012,12(18):3392.
• [6]Jokerst J C,Adkins J A,Bisha B,et al.Development of a Paper-Based Analytical Device for Colorimetric Detection of Select Foodborne Pathogens[J].Anal Chem,2012,84(6):2900.
• [7]de Oliveira R A G,Camargo F,Pesquero N C,et al.A simple method to produce 2D and 3D microfluidic paper-based analytical devices for clinical analysis[J].Anal Chim Acta,2017,957(C):40.
• [8]Cheng C M,Martinez A W,Gong J,et al.Paper-based ELISA[J].Angew Chem Int Ed Engl,2010,49(28):4771.
• [9]Kaewarsa P,Laiwattanapaisal W,Palasuwan A,et al.A new paper-based analytical device for detection of glucose-6-phosphate dehydrogenase deficiency[J].Talanta,2017,164:534.
• [10]Kang S M,Jang S C,Haldorai Y,et al.Facile fabrication of paper-based analytical devices for rapid and highly selective colorimetric detection of cesium in environmental samples[J].RSC Advances,2017,7(76):48374.
• [11]Mazzu Nascimento T,Morbioli G G,Milan L A,et al.Improved assessment of accuracy and performance indicators in paper-based ELISA[J].Anal Methods,2017,9(18):2644.
• [12]程翼宇,瞿海斌,龚行楚.一种纸张的疏水改性方法:中国,201710432902.6[P].2017-06-09.
• [13]Sameenoi Y,Nongkai P N,Nouanthavong S,et al.One-step polymer screen-printing for microfluidic paper-based analytical device(μPAD)fabrication[J].Analyst,2014,139(24):6580.
• [14]曹文杰,苏李娜,樊官伟.丹红注射液药理作用及临床应用研究[J].现代中西医结合杂志,2015(3):335.
• [15]杨静,江振作,柴欣,等.中药注射液“Q-Markers”的辨析研究——丹红注射液研究实例[J].世界科学技术——中医药现代化,2016(12):2056.
• [16]汪悦,邵青,瞿海斌,等.丹红注射液多元指纹图谱及多成分定量分析研究[J].中草药,2014(4):490.
• [17]Liu H T,Wang Y F,Olaleye O,et al.Characterization of in vivo antioxidant constituents and dual-standard quality assessment of Danhong injection[J].Biomed Chromatog,2013,27(5):655.
• [18]韦献雅,殷丽琴,钟成,等.DPPH法评价抗氧化活性研究进展[J].食品科学,2014(9):317.