中国中药杂志

2011, v.36(06) 687-690

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SEMAC快速发现白芍吸收成分群的研究
Quick finding of absorption ingredients of Paeoniae Radix Alba by SEMAC

谭琴;王维皓;宋蓓;陈两绵;朱晶晶;王智民;崔九成;
TAN Qin1,2,3,WANG Weihao1,2,SONG Bei1,2,3,Chen Liangmian1,2,ZHU Jingjing1,2*,WANG Zhimin1,2,CUI Jiucheng3(1.Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China; 2.National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines,Beijing 100700,China; 3.Shaanxi University of Chinese Medicine,Shaanxi 712046,China)

摘要(Abstract):

目的:建立基于肠吸收技术以快速发现白芍吸收成分群的方法,为选择白芍质量控制指标提供参考。方法:采用大鼠肠外翻模型,收集3个质量浓度白芍提取物给药后不同时间的肠囊液样品,用HPLC进行检测分析,并计算其有效成分芍药内酯苷、芍药苷的累计吸收量。结果:白芍中5种主要成分均可进入肠囊,在45 min后能全部检测到;不同质量浓度白芍中芍药内酯苷、芍药苷在各肠段均为线性吸收,R2均大于0.9,符合零级吸收速率;芍药内酯苷和芍药苷在空肠、回肠中的吸收速率常数(Ka)随着白芍提取物浓度增加而增加(P<0.05),符合被动吸收。结论:基于肠吸收的质量评价指标选择方法(SEMAC)可以用于白芍吸收成分群的研究。肠囊对药物成分吸收有选择性,回肠与空肠相比可更多、更快地给出吸收成分信息,选择肠外翻60 min的样品检测白芍吸收成分比较适宜。
Objective: To establish a method for quick finding of the absorption ingredients of Paeoniae Radix Alba in order to select the index of quality control.Method:The absorption ingredients of three concentration of Paeoniae Radix Alba were investigated with the in vitro-everted intestinal sac(VEIS) model.The intestinal sac fluids of jejunum and ileum were collected in different time and detected by HPLC.The accumulative absorption quantity of albiflorin and paeoniflorin were calculated,respectively.Result: Five ingredients could be detected.In different concentrations of Paeoniae Radix Alba,albiflorin and paeoniflorin in various intestinal sections were the linear absorption(R2>0.9),conformed to the zero order absorption rate.The values of Ka in the jejunum and ileum were increased along with the raised dosage of the Paeoniae Radix Alba(P<0.05),indicating a passive absorption manner.Conclusion: SEMAC could be used as a tool to find the absorption ingredients of Paeoniae Radix Alba.Compared with the jejunum,the ileum could provide the more absorption information.It was showed that the optimal detecting time was 60 min.

关键词(KeyWords): 白芍;肠外翻技术;HPLC;吸收成分
Paeoniae Radix Alba;selection of evaluation markers by absorption components(SEMAC);HPLC;absorption ingredients

Abstract:

Keywords:

基金项目(Foundation): 国家“重大新药创制”科技重大专项(2009ZX09308-003,2009ZX09301-005,2009ZX09502-021);; 中医药行业科技专业资助项目(200707009)

作者(Author): 谭琴;王维皓;宋蓓;陈两绵;朱晶晶;王智民;崔九成;
TAN Qin1,2,3,WANG Weihao1,2,SONG Bei1,2,3,Chen Liangmian1,2,ZHU Jingjing1,2*,WANG Zhimin1,2,CUI Jiucheng3(1.Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China; 2.National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines,Beijing 100700,China; 3.Shaanxi University of Chinese Medicine,Shaanxi 712046,China)

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